Rem2 signaling affects neuronal structure and function in part by regulation of gene expression

Mol Cell Neurosci. 2017 Dec:85:190-201. doi: 10.1016/j.mcn.2017.10.004. Epub 2017 Oct 21.

Abstract

The central nervous system has the remarkable ability to convert changes in the environment in the form of sensory experience into long-term alterations in synaptic connections and dendritic arborization, in part through changes in gene expression. Surprisingly, the molecular mechanisms that translate neuronal activity into changes in neuronal connectivity and morphology remain elusive. Rem2, a member of the Rad/Rem/Rem2/Gem/Kir (RGK) subfamily of small Ras-like GTPases, is a positive regulator of synapse formation and negative regulator of dendritic arborization. Here we identify that one output of Rem2 signaling is the regulation of gene expression. Specifically, we demonstrate that Rem2 signaling modulates the expression of genes required for a variety of cellular processes from neurite extension to synapse formation and synaptic function. Our results highlight Rem2 as a unique molecule that transduces changes in neuronal activity detected at the cell membrane to morphologically relevant changes in gene expression in the nucleus.

Keywords: Activity-regulated; Excitatory synapse; Glypican-5 (Gpc5); Lrrtm4; Rem2.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Brain / embryology
  • Brain / metabolism
  • Cells, Cultured
  • Gene Expression Regulation / physiology*
  • Gene Knockout Techniques
  • Mice
  • Monomeric GTP-Binding Proteins / metabolism*
  • Neurogenesis / physiology*
  • Neurons / cytology*
  • Neurons / metabolism*
  • Signal Transduction / physiology

Substances

  • Monomeric GTP-Binding Proteins
  • Rem2 protein, mouse