Chemo-Enzymatic Epoxidation of Lallemantia IbericaSeed Oil: Process Development and Economic-Ecological Evaluation

Appl Biochem Biotechnol. 2018 May;185(1):13-33. doi: 10.1007/s12010-017-2630-1. Epub 2017 Oct 25.

Abstract

The chemo-enzymatic epoxidation of Lallemantia iberica seed oil (LISO), a novel plant oil characterized by its exceptional high content of alpha-linolenic acid (> 60%), was developed using an immobilized lipase from Pseudozyma antarctica and hydrogen peroxide as oxidant. A statistical approach was used to study the effect of enzyme amount, temperature, time, and solvent amount on the oxirane oxygen content obtained during epoxidation. An oxirane oxygen content of 8.6 ± 0.2% corresponding to a yield of 82% was obtained under optimized conditions that were identified to be at an enzyme load of 8.2 g/mol of double bonds, a solvent amount of 56.4 wt.%, a temperature of 33 °C, and an incubation time of 17 h. In addition, the experimental investigation was combined with a techno-economic and ecological assessment gaining detailed information regarding cost structure and environmental impact for the chemo-enzymatic epoxidation of the novel plant oil.

Keywords: Biorefinery; Enzyme; Epoxidation; Lallemantia iberica; Life cycle assessment; Lipase; Plant oil; Techno-economic assessment.

MeSH terms

  • Fungal Proteins / chemistry*
  • Hydrogen Peroxide / chemistry
  • Lamiaceae / chemistry*
  • Lipase / chemistry*
  • Oxidation-Reduction
  • Plant Oils / chemistry*
  • Seeds / chemistry*
  • Ustilaginales / enzymology*

Substances

  • Fungal Proteins
  • Plant Oils
  • Hydrogen Peroxide
  • Lipase