Abstract
Polymeric, ceramic and hybrid material-based three-dimensional (3D) scaffold or matrix structures are important for successful tissue engineering. While the number of approaches utilizing the use of cell-based scaffold and matrix structures is constantly growing, it is essential to provide a framework of their typical preparation and evaluation for tissue engineering. This chapter describes the fabrication of 3D scaffolds using two-photon polymerization, decellularization and cell encapsulation methods and easy-to-use protocols allowing assessing the cell morphology, cytotoxicity and viability in these scaffolds.
Keywords:
Decellularization; Fibrin gel; MTT-assay; Microscopy; Scaffolds; Two-photon polymerization.
Publication types
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Research Support, Non-U.S. Gov't
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Review
MeSH terms
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Bone and Bones / metabolism
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Bone and Bones / ultrastructure
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Cartilage, Articular / metabolism
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Cartilage, Articular / ultrastructure
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Cell Culture Techniques
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Cell Survival / drug effects
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Cells, Immobilized
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Chitosan / chemistry
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Chitosan / pharmacology
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Extracellular Matrix / metabolism
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Extracellular Matrix / ultrastructure
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Fibrin Tissue Adhesive / chemistry
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Humans
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Imaging, Three-Dimensional / instrumentation
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Imaging, Three-Dimensional / methods*
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Lactic Acid / chemistry
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Lactic Acid / pharmacology
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Microscopy, Confocal / instrumentation
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Microscopy, Confocal / methods*
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Polyglycolic Acid / chemistry
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Polyglycolic Acid / pharmacology
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Polyhydroxyethyl Methacrylate / chemistry
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Polyhydroxyethyl Methacrylate / pharmacology
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Polylactic Acid-Polyglycolic Acid Copolymer
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Stem Cells / drug effects
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Stem Cells / metabolism
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Stem Cells / ultrastructure*
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Tissue Engineering / methods*
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Tissue Scaffolds*
Substances
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Fibrin Tissue Adhesive
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Polylactic Acid-Polyglycolic Acid Copolymer
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Polyhydroxyethyl Methacrylate
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Polyglycolic Acid
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Lactic Acid
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Chitosan