Current proteomic studies are generally focused on the most abundant proteoforms encoded by canonical nucleic sequences. Transcriptomic and proteomic data, accumulated in a variety of postgenome sources and coupled with state-of-art analytical technologies, allow to start the identification of aberrant (non-canonical) proteoforms. The main sources of aberrant proteoforms are alternative splicing, single nucleotide polymorphism, and post-translational modifications. The aim of this work was to estimate the heterogeneity of HepG2 proteome. We suggested multiomics approach, which combines transcriptomic (RNAseq) and proteomic (2DE-MS/MS) methods, as a promising strategy to explore the proteome.
Na segodniashniĭ moment issledovaniia v oblasti proteomiki sosredotocheny v osnovnom vokrug naibolee predstavlennykh form belkov, zachastuiu kodiruemykh kanonicheskimi (neizmenennymi) nukleotidnymi posledovatel'nostiami. Nakoplennyĭ massiv transkriptomnykh i proteomnykh dannykh nariadu s vysokim urovnem sovremennykh tekhnologicheskikh vozmozhnosteĭ postgenomnykh issledovaniĭ pozvoliaet pristupit' k identifikatsii aberrantnykh form belkov. Dannaia rabota byla natselena na otsenku geterogennosti proteoma HepG2, voznikaiushcheĭ v rezul'tate realizatsii aberratsiĭ na belkovom urovne. V kachestve perspektivnogo instrumenta issledovaniia proteoma byl predlozhen kompleks transkriptomnykh (RNAseq) i proteomnykh (2DE i MS/MS) metodov.
Keywords: alternative splicing; post-translational modification; proteoform; proteome; single amino acid polymorphism; transcriptome; transcriptoproteome.