ViewBS: a powerful toolkit for visualization of high-throughput bisulfite sequencing data

Bioinformatics. 2018 Feb 15;34(4):708-709. doi: 10.1093/bioinformatics/btx633.

Abstract

Motivation: High throughput bisulfite sequencing (BS-seq) is an important technology to generate single-base DNA methylomes in both plants and animals. In order to accelerate the data analysis of BS-seq data, toolkits for visualization are required.

Results: ViewBS, an open-source toolkit, can extract and visualize the DNA methylome data easily and with flexibility. By using Tabix, ViewBS can visualize BS-seq for large datasets quickly. ViewBS can generate publication-quality figures, such as meta-plots, heat maps and violin-boxplots, which can help users to answer biological questions. We illustrate its application using BS-seq data from Arabidopsis thaliana.

Availability: ViewBS is freely available at: https://github.com/xie186/ViewBS.

Contact: [email protected].

Supplementary information: Supplementary data are available at Bioinformatics online.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • DNA Methylation*
  • High-Throughput Nucleotide Sequencing / methods*
  • Plants / genetics
  • Plants / metabolism
  • Sequence Analysis, DNA / methods*
  • Software*
  • Sulfites

Substances

  • Sulfites
  • hydrogen sulfite