Objective To investigate the polymorphisms of 23 Y-STR loci in a Han population in Jiangsu province. Methods Blood samples were collected from 4821 unrelated healthy Han males in Jiangsu province. DNA templates were amplified by PowerPlex Y23 kit,and the amplification products were detected by 3500xL genetic analyzer. Then,we calculated the allele frequencies and gene diversities respectively,as well as the haplotype frequencies and haplotype diversities. Results The gene diversity of these 23 Y-STR loci ranged 0.4099-0.9696. A total of 4781 haplotypes were detected,of which 4743 were found once. The haplotype diversity was 0.99999812. Conclusion The 23 Y-STR loci used in this study are highly polymorphic in Han individuals in Jiangsu province and therefore suitable for population genetic study and forensic individual identification.
目的 调查23个Y染色体STR基因座在江苏汉族人群中的多态性分布。方法 采用PowerPlex Y23荧光标记复合扩增系统,对江苏地区4821名汉族无关男性个体的静脉血DNA进行复合扩增,3500xL遗传分析仪进行毛细管电泳检测,分别计算各个STR基因座的等位基因频率和基因多样性,以及23个基因座联合构成的单倍型频率和单倍型多样性。结果 23个Y染色体STR基因座在4821名江苏汉族男性个体中的基因多样性值介于0.4099~0.9696。共检出4781种不同的单倍型,其中有4743种只出现1次,单倍型多样性达到0.99999812。结论 23个Y染色体STR基因座联合应用于江苏地区汉族人群时具有较好的遗传多态性分布,适用于该地区的群体遗传学与法医学个体识别等研究。.