Reduced IL-6 levels and tumor-associated phospho-STAT3 are associated with reduced tumor development in a mouse model of lung cancer chemoprevention with myo-inositol

Int J Cancer. 2018 Apr 1;142(7):1405-1417. doi: 10.1002/ijc.31152. Epub 2017 Dec 23.

Abstract

Several promising chemopreventive agents have for lung cancer emerged in preclinical models and in retrospective trials. These agents have been shown to modulate pathways altered in carcinogenesis and reduce markers of carcinogenesis in animal and cell culture models. Cancer-prone transgenic mice with oncogenic Kras expressed in the airway epithelium (CcspCre/+ ; KrasLSL-G12D/+ ) were raised on diets compounded with myo-inositol. These animals form lung premalignant lesions in a stereotypical fashion over the ten weeks following weaning. Mice raised on myo-inositol containing diets showed potent reduction in the number, size, and stage of lesions as compared to those raised on control diets. myo-inositol has previously been reported to inhibit phosphoinositide 3-kinase (PI3K) signaling. However, in mice raised on myo-inositol, total PI3K signaling was largely unaffected. Proteomic and cytokine analyses revealed large reduction in IL-6 related pathways, including STAT3 phosphorylation. This effect was not due to direct inhibition of IL-6 production and autocrine signaling within the tumor cell, but rather through alteration in macrophage recruitment and in phenotype switching, with an increase in antitumoral M1 macrophages.

Keywords: IL-6; Kras; chemoprevention; lung cancer; lung cancer chemoprevention; myo-inositol.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Anticarcinogenic Agents / pharmacology
  • Disease Models, Animal
  • Humans
  • Inositol / pharmacology*
  • Interleukin-6 / metabolism*
  • Lung Neoplasms / pathology*
  • Macrophages / drug effects*
  • Mice
  • Mice, Transgenic
  • Phosphorylation
  • STAT3 Transcription Factor / metabolism*

Substances

  • Anticarcinogenic Agents
  • Interleukin-6
  • STAT3 Transcription Factor
  • Stat3 protein, mouse
  • interleukin-6, mouse
  • Inositol