Alternative animal model for studies of total skin thickness burns

Acta Cir Bras. 2017 Oct;32(10):836-842. doi: 10.1590/s0102-865020170100000005.

Abstract

Purpose: To present an alternative experimental model of third degree burn of easy reproducibility.

Methods: Eighteen male Wister rats were randomly divided into three groups, 6 of which were allocated to each group. A soldering iron coupled to an aluminum plate was used to produce burn, at a temperature of 150ºC, with different exposure times per group. Group 5 (G5) animals were burned at 150°C with exposure time of 5 seconds; Group 10 (G10) the animals were burned at 150°C with exposure time of 10 seconds and group 15 (G15) the animals were burned at 150°C with exposure time of 15 seconds.

Results: Histopathological analyzes showed that all three groups had similar morphological characteristics, with total thickness involvement.

Conclusion: The technique is effective to reproduce a third degree burn and suggests the temperature of 150ºC with 5 seconds of exposure in order to minimize the risks to the animals.

MeSH terms

  • Animals
  • Burns / pathology*
  • Disease Models, Animal*
  • Male
  • Random Allocation
  • Rats
  • Rats, Wistar
  • Severity of Illness Index
  • Skin / pathology*