Objective: To observe the effects of deguelin on the proliferation of breast cancer MCF-7 cells and lung cancer H1299 cells in vitro and the expression of minichromosome maintenance protein 3 (MCM3) and CDC45 in the cells.
Methods: MTT assay was used to evaluate the proliferation of MCF-7 and H1299 cells exposed to different concentrations of deguelin for 48, 72 or 96 h. The growth of the cells was observed microscopically and the changes of MCM3 and CDC45 expressions in MCF-7 and H1299 cells following deguelin treatment were detected with fluorescence quantitative PCR.
Results: The proliferation of MCF-7 cells was significantly inhibited by exposure to 0.25, 0.5, 1, 5, 10, 30, and 50 µmol/L deguelin for 48, 72, and 96 h in a concentration- and time-dependent manner. In MCF-7 cells, the IC50 of deguelin at 48, 72, and 96 h was 9, 3, and 2 µmol/L, respectively. Deguelin treatments of H1299 cells at 0.5, 1, 5, 10, 30, 50, and 100 µmol/L also resulted in a concentration- and time-dependent inhibition of the cell growth with an IC50 at 96 h of 2 µmol/L. Optical microscopy of the cells revealed a decreased number of viable cells with obvious cell shrinkage following deguelin treatments. The expression of MCM3 and CDC45 were significantly reduced in the cells after deguelin treatments.
Conclusion: Deguelin can inhibit the proliferation of MCF-7 and H1299 cells in vitro and down-regulate the expression of MCM3 and CDC45 in the cells.
目的: 探究鱼藤素对MCF-7及H1299细胞的增殖作用，并从mRNA水平研究了鱼藤素对微小染色体维系蛋白3（MCM3）和CDC45在MCF-7及H1299细胞中的影响。
方法: 利用MTT法研究不同浓度的鱼藤素分别处理MCF-7和H1299细胞48、72、96 h后的抑制增殖作用；利用显微镜观察MCF-7和H1299细胞的生长状态；荧光定量PCR探究鱼藤素对MCF-7和H1299细胞中MCM3-CDC45表达的变化。
结果: 0.25、0.5、1、5、10、30、50 μmol/L的鱼藤素作用MCF-7细胞48、72、96 h后，能明显抑制其增殖，且呈浓度时间依赖性，鱼藤素作用MCF-7 48、72、96 h的IC50分别为9、3、2 μmol/L；0.5、1、5、10、30、50、100 μmol/L的鱼藤素作用H1299细胞48、72、96 h，抑制率呈浓度时间依赖性，鱼藤素作用H1299细胞96 h的IC50为2 μmol/L。光学显微镜下，观察到药物处理组中，细胞数目减少，形态皱缩明显。鱼藤素干预MCF-7及H1299细胞，MCM3-CDC45的表达量减少。
结论: 鱼藤素可抑制MCF-7、H1299细胞增殖，并可下调细胞中MCM3和CDC45的表达。