Correcting CRISPR for copy number

John Paul Shen; Trey Ideker

doi:10.1038/ng.3994

Correcting CRISPR for copy number

Nat Genet. 2017 Nov 29;49(12):1674-1675. doi: 10.1038/ng.3994.

Authors

John Paul Shen¹, Trey Ideker¹

Affiliation

¹ Department of Medicine, University of California, San Diego, La Jolla, California, USA.

PMID: 29186130
DOI: 10.1038/ng.3994

Abstract

The CRISPR-Cas9 system enables global screens of gene function with high sensitivity and specificity, but off-target effects have been reported for CRISPR guide RNAs targeting genes that are amplified at high copy number. A new study describes a computational approach to correct for this copy number effect, increasing the specificity of CRIPSR screens to identify essential genes.

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MeSH terms

CRISPR-Cas Systems*
Clustered Regularly Interspaced Short Palindromic Repeats*
DNA Copy Number Variations
Genes, Essential
RNA, Guide, CRISPR-Cas Systems
Sensitivity and Specificity

Substances

RNA, Guide, CRISPR-Cas Systems