Chlamydia trachomatis antibody detection in home-collected blood samples for use in epidemiological studies

B M Hoenderboom; E F van Ess; I V F van den Broek; I H M van Loo; C J P A Hoebe; S Ouburg; S A Morré

doi:10.1016/j.mimet.2017.11.022

Chlamydia trachomatis antibody detection in home-collected blood samples for use in epidemiological studies

J Microbiol Methods. 2018 Jan:144:164-167. doi: 10.1016/j.mimet.2017.11.022. Epub 2017 Nov 28.

Authors

B M Hoenderboom¹, E F van Ess², I V F van den Broek³, I H M van Loo⁴, C J P A Hoebe⁵, S Ouburg², S A Morré⁶

Affiliations

¹ Epidemiology and Surveillance Unit, Centre for Infectious Disease Control, National Institute for Public Health and the Environment, Bilthoven, The Netherlands; Laboratory of Immunogenetics, Department Medical Microbiology and Infection Control, VU University Medical Center, Amsterdam, The Netherlands. Electronic address: [email protected].
² Laboratory of Immunogenetics, Department Medical Microbiology and Infection Control, VU University Medical Center, Amsterdam, The Netherlands.
³ Epidemiology and Surveillance Unit, Centre for Infectious Disease Control, National Institute for Public Health and the Environment, Bilthoven, The Netherlands.
⁴ Department of Medical Microbiology, Care and Public Health Research Institute (CAPHRI), Maastricht University Medical Centre (MUMC+), Maastricht, The Netherlands.
⁵ Department of Medical Microbiology, Care and Public Health Research Institute (CAPHRI), Maastricht University Medical Centre (MUMC+), Maastricht, The Netherlands; Department of Sexual Health, Infectious Diseases and Environmental Health, South Limburg Public Health Service (GGD South Limburg), Geleen, The Netherlands.
⁶ Laboratory of Immunogenetics, Department Medical Microbiology and Infection Control, VU University Medical Center, Amsterdam, The Netherlands; Institute for Public Health Genomics (IPHG), Department of Genetics and Cell Biology, Research School GROW (School for Oncology & Developmental Biology), Faculty of Health, Medicine & Life Sciences, University of Maastricht, Maastricht, The Netherlands.

PMID: 29196272
DOI: 10.1016/j.mimet.2017.11.022

Abstract

Capillary blood collected in serum tubes was subjected to centrifugation delay while stored at room temperature. Chlamydia trachomatis (CT) IgG concentrations in aliquoted serum of these blood samples remained stable for seven days after collection. CT IgG concentrations can reliably be measured in mailed blood samples in epidemiological studies.

Keywords: Chlamydia IgG stability; Home-collected blood samples; Separation delay of whole blood.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antibodies, Bacterial / blood*
Antibodies, Bacterial / isolation & purification*
Bacteriological Techniques / methods
Blood Chemical Analysis / instrumentation
Blood Chemical Analysis / methods
Blood Preservation / methods
Blood Specimen Collection / instrumentation
Blood Specimen Collection / methods*
Chlamydia trachomatis / immunology*
Epidemiologic Studies*
Humans
Immunoglobulin G / blood
Immunoglobulin G / isolation & purification
Sensitivity and Specificity
Time Factors

Substances

Antibodies, Bacterial
Immunoglobulin G