Use of Proteins Identified through a Functional Genomic Screen To Develop a Protein Subunit Vaccine That Provides Significant Protection against Virulent Streptococcus suis in Pigs

Infect Immun. 2018 Feb 20;86(3):e00559-17. doi: 10.1128/IAI.00559-17. Print 2018 Mar.

Abstract

Streptococcus suis is a bacterium that is commonly carried in the respiratory tract and that is also one of the most important invasive pathogens of swine, commonly causing meningitis, arthritis, and septicemia. Due to the existence of many serotypes and a wide range of immune evasion capabilities, efficacious vaccines are not readily available. The selection of S. suis protein candidates for inclusion in a vaccine was accomplished by identifying fitness genes through a functional genomics screen and selecting conserved predicted surface-associated proteins. Five candidate proteins were selected for evaluation in a vaccine trial and administered both intranasally and intramuscularly with one of two different adjuvant formulations. Clinical protection was evaluated by subsequent intranasal challenge with virulent S. suis While subunit vaccination with the S. suis proteins induced IgG antibodies to each individual protein and a cellular immune response to the pool of proteins and provided substantial protection from challenge with virulent S. suis, the immune response elicited and the degree of protection were dependent on the parenteral adjuvant given. Subunit vaccination induced IgG reactive against different S. suis serotypes, indicating a potential for cross protection.

Keywords: Streptococcus suis; adjuvant; functional genomics; subunit; vaccine.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Bacterial / immunology
  • Bacterial Proteins / administration & dosage
  • Bacterial Proteins / genetics
  • Bacterial Proteins / immunology*
  • Cross Protection
  • Female
  • Genomics
  • Male
  • Streptococcal Infections / immunology
  • Streptococcal Infections / microbiology
  • Streptococcal Infections / prevention & control
  • Streptococcal Infections / veterinary*
  • Streptococcal Vaccines / administration & dosage*
  • Streptococcal Vaccines / genetics
  • Streptococcal Vaccines / immunology
  • Streptococcus suis / chemistry
  • Streptococcus suis / genetics
  • Streptococcus suis / immunology*
  • Streptococcus suis / pathogenicity
  • Swine
  • Swine Diseases / immunology
  • Swine Diseases / microbiology
  • Swine Diseases / prevention & control*
  • Vaccines, Subunit / administration & dosage
  • Vaccines, Subunit / genetics
  • Vaccines, Subunit / immunology
  • Virulence

Substances

  • Antibodies, Bacterial
  • Bacterial Proteins
  • Streptococcal Vaccines
  • Vaccines, Subunit