Three new mutants were induced in a laboratory strain of Anopheles quadrimaculatus (Say), species A, by feeding adult males ethyl methanesulfonate (EMS). Rose eye (ro) is a recessive X-linked trait. Short antenna (Sa) and melanotic (Mel) are dominant autosomal traits that are lethal in the homozygous condition. Linkage crosses and X-autosome translocations were used to assign short antenna to the right arm of chromosome 3 about 45 map units proximal to stripe (st+), and melanotic was located on chromosome 2 near the centromere.