Comparative analyses of DHA-Phosphatidylcholine and recombination of DHA-Triglyceride with Egg-Phosphatidylcholine or Glycerylphosphorylcholine on DHA repletion in n-3 deficient mice

Lipids Health Dis. 2017 Dec 8;16(1):234. doi: 10.1186/s12944-017-0623-2.

Abstract

Background: Docosahexaenoic acid (DHA) is important for optimal neurodevelopment and brain function during the childhood when the brain is still under development.

Methods: The effects of DHA-Phosphatidylcholine (DHA-PC) and the recombination of DHA-Triglyceride with egg PC (DHA-TG + PC) or α-Glycerylphosphorylcholine (DHA-TG + α-GPC) were comparatively analyzed on DHA recovery and the DHA accumulation kinetics in tissues including cerebral cortex, erythrocyte, liver, and testis were evaluated in the weaning n-3 deficient mice.

Results: The concentration of DHA in weaning n-3 deficient mice could be recovered rapidly by dietary DHA supplementation, in which DHA-PC exhibited the better efficacy than the recombination of DHA-Triglyceride with egg PC or α-GPC. Interestingly, DHA-TG + α-GPC exhibited the greater effect on DHA accumulation than DHA-TG + PC in cerebral cortex and erythrocyte (p < 0.05), which was similar to DHA-PC. Meanwhile, DHA-TG + PC showed a similar effect to DHA-PC on DHA repletion in testis, which was better than that of DHA-TG + α-GPC (p < 0.05).

Conclusion: We concluded that different forms of DHA supplements could be applied targetedly based on the DHA recovery in different tissues, although the supplemental effects of the recombination of DHA-Triglyceride with egg PC or α-GPC were not completely equivalent to that of DHA-PC, which could provide some references to develop functional foods to support brain development and function.

Keywords: DHA-Phosphatidylcholine; N-3 Fatty acid deficiency; Repletion; Tissue accretion kinetics; Weaning.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Cerebral Cortex / drug effects
  • Cerebral Cortex / metabolism
  • Chickens
  • Dietary Supplements*
  • Docosahexaenoic Acids / administration & dosage*
  • Docosahexaenoic Acids / chemistry
  • Docosahexaenoic Acids / metabolism
  • Eggs / analysis
  • Erythrocytes / drug effects
  • Erythrocytes / metabolism
  • Fatty Acids, Omega-3 / deficiency*
  • Glycerylphosphorylcholine / administration & dosage*
  • Glycerylphosphorylcholine / chemistry
  • Liver / drug effects
  • Liver / metabolism
  • Male
  • Mice
  • Mice, Inbred ICR
  • Organ Specificity
  • Phosphatidylcholines / administration & dosage*
  • Phosphatidylcholines / chemistry
  • Testis / drug effects
  • Testis / metabolism
  • Tissue Distribution
  • Triglycerides / administration & dosage*
  • Triglycerides / chemistry
  • Weaning

Substances

  • Fatty Acids, Omega-3
  • Phosphatidylcholines
  • Triglycerides
  • Docosahexaenoic Acids
  • Glycerylphosphorylcholine