The influence of adiposity and acute exercise on circulating hepatokines in normal-weight and overweight/obese men

Appl Physiol Nutr Metab. 2018 May;43(5):482-490. doi: 10.1139/apnm-2017-0639. Epub 2017 Dec 8.

Abstract

Hepatokines are liver-secreted proteins with potential to influence glucose regulation and other metabolic parameters. This study investigated differences in adiposity status on 5 novel hepatokines and characterised their response to acute moderate-intensity exercise in groups of normal-weight and overweight/obese men. Twenty-two men were recruited into normal-weight and overweight/obese groups (body mass index: 18.5 to 24.9 and 25.0 to 34.9 kg·m-2). Each completed 2 experimental trials, exercise and control. During exercise trials, participants performed 60 min of moderate-intensity treadmill exercise (∼60% peak oxygen uptake) and then rested for 6 h. Participants rested throughout control trials. Circulating fibroblast growth factor-21 (FGF21), follistatin, leukocyte cell-derived chemotaxin 2 (LECT2), fetuin-A, and selenoprotein-P (SeP) were measured throughout. Fasted (resting) FGF21 and LECT2 were higher in overweight/obese individuals (129% and 55%; P ≤ 0.01) and correlated with indices of adiposity and insulin resistance; whereas circulating follistatin was lower in overweight/obese individuals throughout trial days (17%, P < 0.05). In both groups, circulating concentrations of FGF21 and follistatin were transiently elevated after exercise for up to 6 h (P ≤ 0.02). Circulating fetuin-A and SeP were no different between groups (P ≥ 0.19) and, along with LECT2, were unaffected by exercise (P ≥ 0.06). These findings show that increased adiposity is associated with a modified hepatokine profile, which may represent a novel mechanism linking excess adiposity to metabolic health. Furthermore, acute perturbations in circulating FGF21 and follistatin after exercise may contribute to the health benefits of an active lifestyle.

Keywords: activité physique; foie; insulin resistance; insulinorésistance; liver; obesity; obésité; organokines; physical activity.

MeSH terms

  • Adiposity*
  • Adult
  • Biomarkers / blood
  • Blood Glucose / metabolism
  • Body Composition
  • Body Mass Index
  • Body Weight
  • Case-Control Studies
  • Exercise Test
  • Exercise*
  • Fibroblast Growth Factors / blood
  • Follistatin / blood
  • Glucagon / blood
  • Humans
  • Insulin / blood
  • Insulin Resistance
  • Intercellular Signaling Peptides and Proteins / blood
  • Life Style
  • Male
  • Middle Aged
  • Obesity / blood*
  • Overweight / blood*
  • Selenoprotein P / blood
  • Young Adult
  • alpha-2-HS-Glycoprotein / metabolism

Substances

  • Biomarkers
  • Blood Glucose
  • FST protein, human
  • Follistatin
  • Insulin
  • Intercellular Signaling Peptides and Proteins
  • LECT2 protein, human
  • Selenoprotein P
  • alpha-2-HS-Glycoprotein
  • fibroblast growth factor 21
  • Fibroblast Growth Factors
  • Glucagon