Evaluation of colorimetric assays for determination of H2O2in planta during fungal wood decomposition

J Microbiol Methods. 2018 Feb:145:10-13. doi: 10.1016/j.mimet.2017.12.004. Epub 2017 Dec 11.

Abstract

Hydrogen peroxide (H2O2) plays a critical role in generating oxygen radicals as fungi attack and deconstruct plant cell walls. Its concentrations in planta, however, are often low during decomposition and evade detection by traditional methods. Here, we compared relevant methods and selected the best based on detection limits and selectivity.

Keywords: Amplex red 10; Brown rot; Fungi; Radical.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Ampyrone / chemistry
  • Ampyrone / pharmacology
  • Benzothiazoles / chemistry
  • Benzothiazoles / pharmacology
  • Cell Wall / drug effects
  • Cellulose / chemistry
  • Cellulose / metabolism
  • Horseradish Peroxidase / chemistry
  • Horseradish Peroxidase / pharmacology
  • Hydrogen Peroxide / analysis*
  • Hydrogen Peroxide / chemistry
  • Hydrogen Peroxide / metabolism
  • Limit of Detection
  • Oxazines / chemistry
  • Oxazines / pharmacology
  • Phenols / chemistry
  • Phenols / pharmacology
  • Polyporales / chemistry
  • Polyporales / metabolism*
  • Reactive Oxygen Species / chemistry
  • Reactive Oxygen Species / metabolism
  • Sulfonic Acids / chemistry
  • Sulfonic Acids / pharmacology
  • Sulfoxides / chemistry
  • Sulfoxides / pharmacology
  • Wood / microbiology*

Substances

  • Benzothiazoles
  • Oxazines
  • Phenols
  • Reactive Oxygen Species
  • Sulfonic Acids
  • Sulfoxides
  • Ampyrone
  • Amplex Red
  • 2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid
  • Cellulose
  • Hydrogen Peroxide
  • Horseradish Peroxidase
  • xylenol orange