Anti-proliferative activity of a purified polysaccharide isolated from the basidiomycete fungus Paxillus involutus

Ankush Barad; Sebastian Mackedenski; Wai Ming Li; Xiao Jie Li; Bryan Chu Chwen Lim; Faran Rashid; Linda E Tackaberry; Hugues B Massicotte; Keith N Egger; Kerry Reimer; Peter C K Cheung; Chow H Lee

doi:10.1016/j.carbpol.2017.11.058

Anti-proliferative activity of a purified polysaccharide isolated from the basidiomycete fungus Paxillus involutus

Carbohydr Polym. 2018 Feb 1:181:923-930. doi: 10.1016/j.carbpol.2017.11.058. Epub 2017 Nov 16.

Authors

Affiliations

¹ Chemistry Program, University of Northern British Columbia, Prince George, British Columbia, Canada. Electronic address: [email protected].
² Chemistry Program, University of Northern British Columbia, Prince George, British Columbia, Canada. Electronic address: [email protected].
³ Chemistry Program, University of Northern British Columbia, Prince George, British Columbia, Canada. Electronic address: [email protected].
⁴ Food and Nutritional Sciences Program, School of Life Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China. Electronic address: [email protected].
⁵ Chemistry Program, University of Northern British Columbia, Prince George, British Columbia, Canada. Electronic address: [email protected].
⁶ Chemistry Program, University of Northern British Columbia, Prince George, British Columbia, Canada. Electronic address: [email protected].
⁷ Ecosystem Science and Management Program, University of Northern British Columbia, Prince George, British Columbia, Canada. Electronic address: [email protected].
⁸ Ecosystem Science and Management Program, University of Northern British Columbia, Prince George, British Columbia, Canada. Electronic address: [email protected].
⁹ Ecosystem Science and Management Program, University of Northern British Columbia, Prince George, British Columbia, Canada. Electronic address: [email protected].
¹⁰ Chemistry Program, University of Northern British Columbia, Prince George, British Columbia, Canada. Electronic address: [email protected].
¹¹ Food and Nutritional Sciences Program, School of Life Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China. Electronic address: [email protected].
¹² Chemistry Program, University of Northern British Columbia, Prince George, British Columbia, Canada. Electronic address: [email protected].

PMID: 29254055
DOI: 10.1016/j.carbpol.2017.11.058

Abstract

A growth-inhibitory polysaccharide (GIPinv) was purified using size-exclusion and ion-exchange chromatography from the fourth sodium hydroxide extraction step of a fungus found in British Columbia. The fungus was genetically identified as a member of the Paxillus involutus complex. GIPinv has an average molecular weight of 229kDa and is a heteroglycan composed of glucose (65.9%), galactose (20.8%), mannose (7.8%), fucose (3.2%) and xylose (2.3%). GC-MS methylation analysis suggests that GIPinv has mixed linkages in the backbone containing (1→6)-Gal (25.5%), (1→4)-Glc (18.3%), (1→6)-Glc (8.3%), (1→3)-Glc (5.3%) and (1→2)-Xyl (4.5%). GIPinv has branching points at (1→2, 6)-Man (8.6%) and (1→3, 6)-Man (4.9%) having unsubstituted fucose (8.3%) and glucose (16.3%) as terminal sugars. GIPinv had growth-inhibitory activity against several cancer cell lines and triggered apoptosis. GIPinv should be further explored as a potential anti-cancer agent and a unique polysaccharide.

Keywords: British Columbia; Growth inhibition; Mushroom; Paxillus involutus; Polysaccharide.

MeSH terms

Apoptosis / drug effects
Basidiomycota / chemistry*
Cell Line, Tumor
Cell Proliferation / drug effects
Chemical Fractionation
Complex Mixtures
Gas Chromatography-Mass Spectrometry
Humans
Monosaccharides / analysis
Phylogeny
Polysaccharides / isolation & purification*
Polysaccharides / pharmacology*

Substances

Complex Mixtures
Monosaccharides
Polysaccharides