Characterization of KPC-Encoding Plasmids from Enterobacteriaceae Isolated in a Czech Hospital

Rudolf Kukla; Katerina Chudejova; Costas C Papagiannitsis; Matej Medvecky; Katerina Habalova; Lenka Hobzova; Radka Bolehovska; Lenka Pliskova; Jaroslav Hrabak; Helena Zemlickova

doi:10.1128/AAC.02152-17

Characterization of KPC-Encoding Plasmids from Enterobacteriaceae Isolated in a Czech Hospital

Antimicrob Agents Chemother. 2018 Feb 23;62(3):e02152-17. doi: 10.1128/AAC.02152-17. Print 2018 Mar.

Authors

Rudolf Kukla^#¹, Katerina Chudejova^#^{2

3}, Costas C Papagiannitsis^{4

2

3}, Matej Medvecky^{5

6}, Katerina Habalova¹, Lenka Hobzova⁷, Radka Bolehovska⁸, Lenka Pliskova⁸, Jaroslav Hrabak^{2

3}, Helena Zemlickova^{1

9}

Affiliations

¹ Department of Clinical Microbiology, University Hospital and Faculty of Medicine in Hradec Kralove, Charles University, Hradec Kralove, Czech Republic.
² Department of Microbiology, Faculty of Medicine and University Hospital in Pilsen, Charles University, Pilsen, Czech Republic.
³ Biomedical Center, Faculty of Medicine, Charles University, Pilsen, Czech Republic.
⁴ Department of Clinical Microbiology, University Hospital and Faculty of Medicine in Hradec Kralove, Charles University, Hradec Kralove, Czech Republic [email protected].
⁵ Veterinary Research Institute, Brno, Czech Republic.
⁶ National Centre for Biomolecular Research, Faculty of Science, Masaryk University, Brno, Czech Republic.
⁷ Department of Hospital Hygiene, University Hospital, Hradec Kralove, Czech Republic.
⁸ Department of Clinical Biochemistry and Diagnostics, University Hospital, Hradec Kralove, Czech Republic.
⁹ National Reference Laboratory for Antibiotics, National Institute of Public Health, Prague, Czech Republic.

^# Contributed equally.

Abstract

Ten Enterobacteriaceae isolates collected in a Czech hospital carried bla_KPC-positive plasmids of different sizes (∼30, ∼45, and ∼80 kb). Sequencing revealed three types of plasmids (A to C) with the Tn4401a transposon. Type A plasmids comprised an IncR backbone and a KPC-2-encoding multidrug resistance (MDR) region. Type B plasmids were derivatives of type A plasmids carrying an IncN3-like segment, while type C plasmids were IncP6 plasmids sharing the same KPC-2-encoding MDR region with type A and B plasmids.

Keywords: Citrobacter freundii; Illumina sequencing; IncR; ST18; Tn4401a.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / therapeutic use
Base Sequence
Carbapenems / therapeutic use
Citrobacter freundii / drug effects
Citrobacter freundii / enzymology
Citrobacter freundii / genetics*
Citrobacter freundii / isolation & purification
Czech Republic / epidemiology
DNA Transposable Elements
Drug Resistance, Multiple, Bacterial / genetics
Enterobacteriaceae Infections / drug therapy
Enterobacteriaceae Infections / epidemiology
Enterobacteriaceae Infections / microbiology
Escherichia coli / drug effects
Escherichia coli / enzymology
Escherichia coli / genetics*
Escherichia coli / isolation & purification
Gene Expression
Hospitals
Humans
Isoenzymes / genetics
Isoenzymes / metabolism
Klebsiella pneumoniae / drug effects
Klebsiella pneumoniae / enzymology
Klebsiella pneumoniae / genetics*
Klebsiella pneumoniae / isolation & purification
Microbial Sensitivity Tests
Morganella morganii / drug effects
Morganella morganii / enzymology
Morganella morganii / genetics*
Morganella morganii / isolation & purification
Open Reading Frames
Plasmids / chemistry*
Plasmids / classification
Plasmids / metabolism
Sequence Analysis, DNA
beta-Lactamases / genetics*
beta-Lactamases / metabolism

Substances

Anti-Bacterial Agents
Carbapenems
DNA Transposable Elements
Isoenzymes
beta-lactamase KPC-2
beta-Lactamases