A new vesicle trafficking regulator CTL1 plays a crucial role in ion homeostasis

Yi-Qun Gao; Jiu-Geng Chen; Zi-Ru Chen; Dong An; Qiao-Yan Lv; Mei-Ling Han; Ya-Ling Wang; David E Salt; Dai-Yin Chao

doi:10.1371/journal.pbio.2002978

A new vesicle trafficking regulator CTL1 plays a crucial role in ion homeostasis

PLoS Biol. 2017 Dec 28;15(12):e2002978. doi: 10.1371/journal.pbio.2002978. eCollection 2017 Dec.

Authors

Yi-Qun Gao^{1

2}, Jiu-Geng Chen¹, Zi-Ru Chen^{1

2}, Dong An¹, Qiao-Yan Lv^{1

2}, Mei-Ling Han^{1

2}, Ya-Ling Wang¹, David E Salt³, Dai-Yin Chao¹

Affiliations

¹ National Key Laboratory of Plant Molecular Genetics, Chinese Academy of Sciences Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
² University of Chinese Academy of Sciences, Beijing, China.
³ Centre for Plant Integrative Biology, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, United Kingdom.

Abstract

Ion homeostasis is essential for plant growth and environmental adaptation, and maintaining ion homeostasis requires the precise regulation of various ion transporters, as well as correct root patterning. However, the mechanisms underlying these processes remain largely elusive. Here, we reported that a choline transporter gene, CTL1, controls ionome homeostasis by regulating the secretory trafficking of proteins required for plasmodesmata (PD) development, as well as the transport of some ion transporters. Map-based cloning studies revealed that CTL1 mutations alter the ion profile of Arabidopsis thaliana. We found that the phenotypes associated with these mutations are caused by a combination of PD defects and ion transporter misregulation. We also established that CTL1 is involved in regulating vesicle trafficking and is thus required for the trafficking of proteins essential for ion transport and PD development. Characterizing choline transporter-like 1 (CTL1) as a new regulator of protein sorting may enable researchers to understand not only ion homeostasis in plants but also vesicle trafficking in general.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adenosine Triphosphatases / metabolism
Arabidopsis / genetics
Arabidopsis / metabolism*
Arabidopsis Proteins / genetics
Arabidopsis Proteins / metabolism
Arabidopsis Proteins / physiology*
Biological Transport
Cation Transport Proteins / metabolism
Cloning, Molecular
Glycoside Hydrolases / genetics
Glycoside Hydrolases / metabolism
Glycoside Hydrolases / physiology*
Homeostasis
Ion Transport / genetics*
Membrane Transport Proteins / genetics
Membrane Transport Proteins / metabolism
Membrane Transport Proteins / physiology*
Mutation
Protein Transport
Symporters / metabolism

Substances

Arabidopsis Proteins
Cation Transport Proteins
HKT1 protein, Arabidopsis
Membrane Transport Proteins
Symporters
choline transporter
CTL1 protein, Arabidopsis
Glycoside Hydrolases
Adenosine Triphosphatases
HMA4 protein, Arabidopsis

Grants and funding

Chinese Academy of Sciences http://english.cas.cn/ (grant number XDPB0402) to D-Y C. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Chinese Thousand Talents Program http://www.1000plan.org/ to D-Y C. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Natural Science Foundation of China http://www.nsfc.gov.cn/ (grant number 31422007) to D-Y C. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.