Optimization for imaging through scattering media for confocal microscopes with divided elliptical apertures

Hengjie Tang; Chenxue Wu; Wei Gong; Yao Zheng; Xinpei Zhu; Jiahao Wang; Ke Si

doi:10.1002/jbio.201700293

Optimization for imaging through scattering media for confocal microscopes with divided elliptical apertures

J Biophotonics. 2018 May;11(5):e201700293. doi: 10.1002/jbio.201700293. Epub 2018 Feb 9.

Authors

Hengjie Tang¹, Chenxue Wu¹, Wei Gong², Yao Zheng^{1

2}, Xinpei Zhu², Jiahao Wang², Ke Si^{1

2}

Affiliations

¹ State Key Laboratory of Modern Optical Instrumentation, College of Optical Science and Engineering, Zhejiang University, Hangzhou, China.
² Key Laboratory of Medical Neurobiology of the Ministry of Health of China, Zhejiang Province Key Laboratory of Neurobiology, Department of Neurobiology, Institute of Neuroscience, Zhejiang University School of Medicine, Hangzhou, China.

PMID: 29286580
DOI: 10.1002/jbio.201700293

Abstract

We develop a confocal system equipped with optimal elliptical apertures to improve axial point spread function and signal-to-background ratio (SBR) for different detector sizes. By adjusting the parameters of the elliptical apertures, the axial half width at half-maximum can be reduced to 4.986 (described in optical coordinates) and SBR can be improved to 0.176. We evaluate this system with the 1951 USAF resolution test chart and the primary cultured neuron from SD rat stained by Map-2, and observe better imaging performance, which indicates the potential applications in biological science.

Keywords: apertures; axial resolution; confocal microscopy; imaging; scattering media.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Image Enhancement / methods*
Microscopy, Confocal*
Neurons / cytology
Rats
Rats, Sprague-Dawley
Scattering, Radiation*
Signal-To-Noise Ratio