The cold tolerance of the northern root-knot nematode, Meloidogyne hapla

Xiaojing Wu; Xiaofeng Zhu; Yuanyuan Wang; Xiaoyu Liu; Lijie Chen; Yuxi Duan

doi:10.1371/journal.pone.0190531

The cold tolerance of the northern root-knot nematode, Meloidogyne hapla

PLoS One. 2018 Jan 2;13(1):e0190531. doi: 10.1371/journal.pone.0190531. eCollection 2018.

Authors

Xiaojing Wu¹, Xiaofeng Zhu¹, Yuanyuan Wang², Xiaoyu Liu³, Lijie Chen¹, Yuxi Duan¹

Affiliations

¹ College of Plant Protection, Shenyang Agricultural University, Shenyang, Liaoning, China.
² College of Biology science and technology, Shenyang Agricultural University, Shenyang, Liaoning, China.
³ College of Science, Shenyang Agricultural University, Shenyang, Liaoning, China.

Abstract

The northern root-knot nematode, Meloidogyne hapla, is one of the most important nematode pathogens occurring in cold regions. It is a sedentary, biotrophic parasites of plants and overwinter in the soil or in diseased roots. This study showed that the cold tolerance for the second-stage juveniles (J2) of M. hapla was moderate with the 50% survival temperature (S50) of -2.22°C and the fatal temperature was -6°C when cooling at 0.5°C min-1. Cryoprotective dehydration significantly enhance cold tolerance of M. hapla J2 with the lowest S50 of -3.28°C after held being at -1°C for 6 h. Moreover, cold shock and cold acclimation had significant effects on the freezing survival of M. hapla J2. The lethal temperature of eggs was -18°C. Therefore, the cold tolerance of M. hapla is sufficiently favorable to withstand winters in cold temperature environments.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adaptation, Physiological*
Animals
Cold Temperature*
Freezing
Tylenchoidea / physiology*

Grants and funding

This work was supported by the National Natural Science Foundation of China (grant numbers: 31471748), Mount Tianzhu Scholar Funding Scheme, Special Fund for Agro-scientific Research in the Public Interest (grant numbers: 201103018).(LJC). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.