Evaluation of truncated G protein delivered by live attenuated Salmonella as a vaccine against respiratory syncytial virus

Puyuan Tian; Di Xu; Zhixiang Huang; Fanjing Meng; Jinheng Fu; Hua Wei; Tingtao Chen

doi:10.1016/j.micpath.2017.12.080

Evaluation of truncated G protein delivered by live attenuated Salmonella as a vaccine against respiratory syncytial virus

Microb Pathog. 2018 Feb:115:299-303. doi: 10.1016/j.micpath.2017.12.080. Epub 2018 Jan 3.

Authors

Puyuan Tian¹, Di Xu², Zhixiang Huang³, Fanjing Meng³, Jinheng Fu⁴, Hua Wei⁵, Tingtao Chen⁶

Affiliations

¹ School of Life Sciences, Nanchang University, Nanchang, Jiangxi 330031, PR China; Institute of Translational Medicine, Nanchang University, Nanchang, Jiangxi 330031, PR China.
² School of Life Sciences, Nanchang University, Nanchang, Jiangxi 330031, PR China.
³ Institute of Translational Medicine, Nanchang University, Nanchang, Jiangxi 330031, PR China.
⁴ Jiangxi-OAI Joint Research Institute, Nanchang University, Nanchang, Jiangxi 330047, PR China.
⁵ School of Life Sciences, Nanchang University, Nanchang, Jiangxi 330031, PR China; State Key Laboratory of Food Science and Technology, Nanchang, Jiangxi 330031, PR China.
⁶ School of Life Sciences, Nanchang University, Nanchang, Jiangxi 330031, PR China; Institute of Translational Medicine, Nanchang University, Nanchang, Jiangxi 330031, PR China. Electronic address: [email protected].

PMID: 29306006
DOI: 10.1016/j.micpath.2017.12.080

Abstract

Human respiratory syncytial virus (RSV) can cause severe acute lower respiratory tract disease leading to numerous hospitalizations and deaths in the infant and elderly populations worldwide, while no vaccine or effective drug is available for RSV infections. In the present study, truncated G protein was successfully expressed both in prokaryotic and eukaryotic system, and high levels of serum IgG in response to truncated G protein were observed both in GD-protein group (intramuscularly with purified GD protein) and GD-VNP20009 group (challenged via the oral route with 1 × 10⁹ CFU of pLIVE-RSV-GD-VNP20009 strains) since 21th day, and GD-VNP20009 significantly reduced the productions of IL-1β, IL-6, and TNF-α, histamine and pathological features caused by the RSV Long strain (P < .01). Our data indicated that Salmonella typhimurium can be used to deliver truncated G DNA vaccine and represents a promising effect to protect host against RSV.

Keywords: Constructed DNA vaccine; Respiratory syncytial virus (RSV); VNP20009.

MeSH terms

Animals
Antibodies, Viral / blood*
Bacterial Vaccines / genetics
Bacterial Vaccines / immunology
Cell Line
Chlorocebus aethiops
Disease Models, Animal
Female
HEK293 Cells
Humans
Immunoglobulin G / blood
Interleukin-1beta / biosynthesis
Interleukin-6 / biosynthesis
Mice
Mice, Inbred C57BL
Respiratory Syncytial Virus Infections / immunology
Respiratory Syncytial Virus Infections / prevention & control*
Respiratory Syncytial Virus Infections / virology
Respiratory Syncytial Virus Vaccines / administration & dosage*
Respiratory Syncytial Virus Vaccines / immunology*
Respiratory Syncytial Viruses / genetics
Respiratory Syncytial Viruses / immunology*
Salmonella typhimurium / genetics
Tumor Necrosis Factor-alpha / biosynthesis
Vaccination
Vero Cells
Viral Envelope Proteins / genetics
Viral Envelope Proteins / immunology*

Substances

Antibodies, Viral
Bacterial Vaccines
IL1B protein, mouse
Immunoglobulin G
Interleukin-1beta
Interleukin-6
Respiratory Syncytial Virus Vaccines
Tumor Necrosis Factor-alpha
VNP 20009
Viral Envelope Proteins
attachment protein G
interleukin-6, mouse