Dedifferentiated liposarcoma composed predominantly of rhabdoid/epithelioid cells: a frequently misdiagnosed highly aggressive variant

Hum Pathol. 2018 Jul:77:20-27. doi: 10.1016/j.humpath.2017.12.025. Epub 2018 Jan 5.

Abstract

Dedifferentiated liposarcoma is one of the most common sarcoma types in adults with a predilection for the retroperitoneum. We have recently encountered 6 cases of DDL composed predominantly of rounded, rhabdoid or epithelioid cells mimicking rhabdoid melanoma, epithelioid rhabdomyosarcoma or undifferentiated carcinoma. Patients were 5 males and one female aged 64 to 81 years (median, 68). Tumors originated in the retroperitoneum (n=5; 3 in the psoas muscle) and deep soft tissue of the thigh (n=1). All 3 patients with follow-up died of metastatic disease within 4 to 8 months. Preoperative biopsy diagnoses never suggested dedifferentiated liposarcoma as a possibility; instead carcinoma, rhabdomyosarcoma and lymphoma were on top of suggestions. Five resected tumors were composed predominantly (70%-100%) of anaplastic rounded to oval rhabdoid cells with prominent central nucleoli and paranuclear rhabdoid inclusions. Bi- and multinucleation was a constant feature. The background stroma showed variable myxoid changes and minor mixed inflammatory cells. Two cases showed homologous dedifferentiation and another had sclerosing spindle cell nodule but a well-differentiated lipomatous component was not seen in any. One biopsied case showed solely monotonous small round blue cells with scattered rhabdoid cells. Immunohistochemistry showed expression of MDM2 (6/6), CDK4 (5/6), pancytokeratin AE/1AE3 (4/6) and diffusely desmin and myogenin (2/6). All cases showed high-level co-amplification of MDM2/CDK4 by in situ hybridization. The SWI/SNF complex components (SMARCB1, SMARCA2, SMARCA4, ARID1A and PBRM1) were intact in all cases. This highly aggressive liposarcoma variant needs to be distinguished from a variety of neoplasms including undifferentiated carcinoma, melanoma, lymphoma, rhabdomyosarcoma and others.

Keywords: Dedifferentiated liposarcoma; Epithelioid; Melanoma; Retroperitoneum; Rhabdoid; Rhabdomyosarcoma; SWI/SNF.

MeSH terms

  • Aged
  • Aged, 80 and over
  • Biomarkers, Tumor / analysis*
  • DNA Helicases / metabolism
  • Diagnosis, Differential
  • Epithelioid Cells / pathology*
  • Female
  • Humans
  • Immunohistochemistry / methods
  • Liposarcoma / diagnosis
  • Liposarcoma / pathology*
  • Male
  • Middle Aged
  • Nuclear Proteins / metabolism
  • Rhabdoid Tumor / diagnosis
  • Rhabdoid Tumor / metabolism
  • Rhabdoid Tumor / pathology*
  • SMARCB1 Protein / metabolism
  • Soft Tissue Neoplasms / diagnosis
  • Soft Tissue Neoplasms / pathology*
  • Transcription Factors / metabolism

Substances

  • Biomarkers, Tumor
  • Nuclear Proteins
  • SMARCB1 Protein
  • SMARCB1 protein, human
  • Transcription Factors
  • SMARCA4 protein, human
  • DNA Helicases