Dramatic Changes in Malaria Population Genetic Complexity in Dielmo and Ndiop, Senegal, Revealed Using Genomic Surveillance

Amy K Bei; Makhtar Niang; Awa B Deme; Rachel F Daniels; Fatoumata D Sarr; Cheikh Sokhna; Cheikh Talla; Joseph Faye; Nafissatou Diagne; Souleymane Doucoure; Souleymane Mboup; Dyann F Wirth; Adama Tall; Daouda Ndiaye; Daniel L Hartl; Sarah K Volkman; Aissatou Toure-Balde

doi:10.1093/infdis/jix580

Dramatic Changes in Malaria Population Genetic Complexity in Dielmo and Ndiop, Senegal, Revealed Using Genomic Surveillance

J Infect Dis. 2018 Jan 30;217(4):622-627. doi: 10.1093/infdis/jix580.

Authors

Amy K Bei^{1

2

3}, Makhtar Niang⁴, Awa B Deme^{2

3}, Rachel F Daniels^{1

5}, Fatoumata D Sarr⁶, Cheikh Sokhna⁷, Cheikh Talla⁶, Joseph Faye⁶, Nafissatou Diagne⁷, Souleymane Doucoure⁷, Souleymane Mboup^{2

8}, Dyann F Wirth^{1

5}, Adama Tall⁶, Daouda Ndiaye³, Daniel L Hartl^{1

5

9}, Sarah K Volkman^{1

5

10}, Aissatou Toure-Balde⁴

Affiliations

¹ Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Boston, Massachusetts.
² Laboratory of Bacteriology and Virology, Le Dantec Hospital, Senegal.
³ Laboratory of Parasitology and Mycology, Faculty of Medicine and Pharmacy, Cheikh Anta Diop University, Senegal.
⁴ Immunology Unit, Institut Pasteur de Dakar, Senegal.
⁵ Broad Institute of Massachusetts Institute of Technology and Harvard, Cambridge.
⁶ Epidemiology Unit, Institut Pasteur de Dakar, Senegal.
⁷ French National Research Institute for Sustainable Development, URMITE, Senegal.
⁸ Institut de Recherche en Santé, de Surveillance Epidemiologique et de Formations, Senegal.
⁹ Department of Organismic and Evolutionary Biology, Harvard University, Cambridge.
¹⁰ School of Nursing and Health Sciences, Simmons College, Boston, Massachusetts.

Abstract

Dramatic changes in transmission intensity can impact Plasmodium population diversity. Using samples from 2 distant time-points in the Dielmo/Ndiop longitudinal cohorts from Senegal, we applied a molecular barcode tool to detect changes in parasite genotypes and complexity of infection that corresponded to changes in transmission intensity. We observed a striking statistically significant difference in genetic diversity between the 2 parasite populations. Furthermore, we identified a genotype in Dielmo and Ndiop previously observed in Thiès, potentially implicating imported malaria. This genetic surveillance study validates the molecular barcode as a tool to assess parasite population diversity changes and track parasite genotypes.

Keywords: Dielmo/Ndiop; Senegal; genomic surveillance; malaria; molecular barcode.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Adolescent
Adult
Child
Child, Preschool
DNA Barcoding, Taxonomic
Female
Genetics, Population*
Genome, Protozoan
Genotype*
Humans
Infant
Longitudinal Studies
Malaria / parasitology*
Male
Plasmodium / classification*
Plasmodium / genetics*
Plasmodium / isolation & purification
Senegal
Young Adult