Abstract
A lambda Charon 4A derivative carrying the outB gene of Bacillus subtilis has been identified by transformation of a B. subtilis mutant temperature-sensitive in spore outgrowth. The cloned region is a single EcoRI fragment 14 kb in length. In addition to outB, the cloned DNA includes at least part of the amyE and aroI loci.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacillus subtilis / genetics*
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Bacteriophage lambda
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Cloning, Molecular*
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Crosses, Genetic
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DNA, Bacterial
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DNA, Recombinant
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DNA, Viral
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Electrophoresis, Agar Gel
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Genes, Bacterial*
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Genetic Markers
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Microscopy, Electron
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Spores, Bacterial
Substances
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DNA, Bacterial
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DNA, Recombinant
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DNA, Viral
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Genetic Markers