OATP1B2 deficiency protects against paclitaxel-induced neurotoxicity

J Clin Invest. 2018 Feb 1;128(2):816-825. doi: 10.1172/JCI96160. Epub 2018 Jan 16.

Abstract

Paclitaxel is among the most widely used anticancer drugs and is known to cause a dose-limiting peripheral neurotoxicity, the initiating mechanisms of which remain unknown. Here, we identified the murine solute carrier organic anion-transporting polypeptide B2 (OATP1B2) as a mediator of paclitaxel-induced neurotoxicity. Additionally, using established tests to assess acute and chronic paclitaxel-induced neurotoxicity, we found that genetic or pharmacologic knockout of OATP1B2 protected mice from mechanically induced allodynia, thermal hyperalgesia, and changes in digital maximal action potential amplitudes. The function of this transport system was inhibited by the tyrosine kinase inhibitor nilotinib through a noncompetitive mechanism, without compromising the anticancer properties of paclitaxel. Collectively, our findings reveal a pathway that explains the fundamental basis of paclitaxel-induced neurotoxicity, with potential implications for its therapeutic management.

Keywords: Cancer; Oncology; Toxins/drugs/xenobiotics; Transport.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antineoplastic Agents / toxicity
  • Biomarkers / metabolism
  • Cell Line, Tumor
  • Genotype
  • HEK293 Cells
  • Humans
  • Hyperalgesia / chemically induced*
  • Hyperalgesia / prevention & control
  • Inhibitory Concentration 50
  • Liver-Specific Organic Anion Transporter 1 / deficiency*
  • Liver-Specific Organic Anion Transporter 1 / genetics*
  • MCF-7 Cells
  • Mice
  • Mice, Inbred DBA
  • Mice, Knockout
  • Mice, Transgenic
  • Organic Anion Transporters / genetics
  • Paclitaxel / toxicity*
  • Peripheral Nervous System Diseases / chemically induced*
  • Peripheral Nervous System Diseases / prevention & control
  • Phenotype
  • Pyrimidines / pharmacology*

Substances

  • Antineoplastic Agents
  • Biomarkers
  • Liver-Specific Organic Anion Transporter 1
  • Organic Anion Transporters
  • Pyrimidines
  • Slco1b2 protein, mouse
  • nilotinib
  • Paclitaxel