Transgenic mice specifically expressing amphiregulin in white adipose tissue showed less adipose tissue mass

Genes Cells. 2018 Mar;23(3):136-145. doi: 10.1111/gtc.12558. Epub 2018 Jan 17.

Abstract

To determine adipocytokines that play a regulatory role during obesity development, we explored the genes that encode growth factors and investigated the physiological functions for adipose tissue development. Here, we isolated amphiregulin (Areg) gene whose expression was significantly up-regulated in obese adipose tissues. Areg mRNA level was positively correlated with macrophage marker gene expression in adipose tissues in vivo. Unexpectedly, Areg transgenic mice showed less adipose tissue mass with increased mRNA expression levels of Tnf-α and peroxisome proliferator-activated receptor γ coactivator 1α (Pgc-1α) and delayed white adipose tissue development during the convalescent stage in a dextran sodium sulfate-induced colitis model. This study showed that Areg mRNA expression was significantly up-regulated in obese adipose tissues and over-expression of Areg in white adipose tissue caused less adipose tissue mass.

Keywords: amphiregulin; obese development; white adipose tissue.

MeSH terms

  • Adipose Tissue, White / metabolism
  • Adipose Tissue, White / pathology*
  • Amphiregulin / genetics
  • Amphiregulin / metabolism*
  • Animals
  • Colitis / chemically induced
  • Colitis / metabolism
  • Colitis / pathology*
  • Dextran Sulfate / toxicity
  • Disease Models, Animal*
  • Female
  • Gene Expression Profiling
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Obesity / physiopathology*
  • Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha / genetics
  • Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha / metabolism
  • Tumor Necrosis Factor-alpha / genetics
  • Tumor Necrosis Factor-alpha / metabolism

Substances

  • Amphiregulin
  • Areg protein, mouse
  • Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha
  • Ppargc1a protein, mouse
  • Tumor Necrosis Factor-alpha
  • Dextran Sulfate