Abstract
We describe a lentivirus-encoded chimeric receptor, termed extracellular vesicle (EV)-internalizing receptor (EVIR), which enables the selective uptake of cancer-cell-derived EVs by dendritic cells (DCs). The EVIR enhances DC presentation of EV-associated tumor antigens to CD8+ T cells primarily through MHCI recycling and cross-dressing. EVIRs should facilitate exploring the mechanisms and implications of horizontal transfer of tumor antigens to antigen-presenting cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antigens, Neoplasm / immunology*
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CD8-Positive T-Lymphocytes / immunology*
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Cells, Cultured
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Colonic Neoplasms / immunology*
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Colonic Neoplasms / metabolism
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Colonic Neoplasms / pathology
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Dendritic Cells / immunology*
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Extracellular Vesicles / immunology
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Extracellular Vesicles / metabolism*
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Female
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Histocompatibility Antigens Class II / immunology
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Mice
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Mice, Inbred C57BL
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Receptors, Chimeric Antigen / metabolism*
Substances
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Antigens, Neoplasm
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Histocompatibility Antigens Class II
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Receptors, Chimeric Antigen