Solid Lipid Nanoparticles Carrying Temozolomide for Melanoma Treatment. Preliminary In Vitro and In Vivo Studies

Int J Mol Sci. 2018 Jan 24;19(2):255. doi: 10.3390/ijms19020255.

Abstract

Aim: To develop an innovative delivery system for temozolomide (TMZ) in solid lipid nanoparticles (SLN), which has been preliminarily investigated for the treatment of melanoma.

Materials and methods: SLN-TMZ was obtained through fatty acid coacervation. Its pharmacological effects were assessed and compared with free TMZ in in vitro and in vivo models of melanoma and glioblastoma.

Results: Compared to the standard free TMZ, SLN-TMZ exerted larger effects, when cell proliferation of melanoma cells, and neoangiogeneis were evaluated. SLN-TMZ also inhibited growth and vascularization of B16-F10 melanoma in C57/BL6 mice, without apparent toxic effects.

Conclusion: SLN could be a promising strategy for the delivery of TMZ, allowing an increased stability of the drug and thereby its employment in the treatment of aggressive malignacies.

Keywords: SLN; melanoma; temozolomide.

MeSH terms

  • Animals
  • Biomarkers
  • Cell Line, Tumor
  • Dacarbazine / administration & dosage
  • Dacarbazine / analogs & derivatives*
  • Dacarbazine / chemistry
  • Disease Models, Animal
  • Drug Stability
  • Female
  • Humans
  • Immunohistochemistry
  • Melanoma / drug therapy
  • Melanoma / metabolism
  • Melanoma / pathology*
  • Melanoma, Experimental
  • Mice
  • Molecular Structure
  • Nanoparticles* / chemistry
  • Nanoparticles* / ultrastructure
  • Neoplastic Stem Cells
  • Temozolomide

Substances

  • Biomarkers
  • Dacarbazine
  • Temozolomide