Whole genome sequencing reveals an outbreak of Salmonella Enteritidis associated with reptile feeder mice in the United Kingdom, 2012-2015

Food Microbiol. 2018 May:71:32-38. doi: 10.1016/j.fm.2017.04.005. Epub 2017 Apr 18.

Abstract

Analysis of whole genome sequencing data uncovered a previously undetected outbreak of Salmonella Enteritidis that had been on-going for four years. Cases were resident in all countries of the United Kingdom and 40% of the cases were aged less than 11 years old. Initial investigations revealed that 30% of cases reported exposure to pet snakes. A case-control study was designed to test the hypothesis that exposure to reptiles or their feed were risk factors. A robust case-definition, based on the single nucleotide polymorphism (SNP) profile, increased the power of the analytical study. Following univariable and multivariable analysis, exposure to snakes was the only variable independently associated with infection (Odds ratio 810 95% CI (85-7715) p < 0.001). Isolates of S. Enteritidis belonging to the outbreak profile were recovered from reptile feeder mice sampled at the retail and wholesale level. Control measures included improved public health messaging at point of sale, press releases and engagement with public health and veterinary counterparts across Europe. Mice destined to be fed to reptiles are not regarded as pet food and are not routinely tested for pathogenic bacteria. Routine microbiological testing to ensure feeder mice are free from Salmonella is recommended.

Keywords: Outbreaks; Salmonellosis; Surveillance; Whole genome sequencing; Zoonotic infections.

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Animals
  • Child
  • Child, Preschool
  • Disease Outbreaks
  • Feeding Behavior
  • Female
  • Genome, Bacterial
  • Humans
  • Infant
  • Male
  • Mice / microbiology*
  • Middle Aged
  • Phylogeny
  • Rats / microbiology
  • Salmonella Infections / epidemiology
  • Salmonella Infections / microbiology*
  • Salmonella Infections / transmission
  • Salmonella enteritidis / classification
  • Salmonella enteritidis / genetics
  • Salmonella enteritidis / isolation & purification*
  • Salmonella enteritidis / physiology
  • Snakes / microbiology*
  • Snakes / physiology
  • United Kingdom / epidemiology
  • Whole Genome Sequencing
  • Young Adult
  • Zoonoses / epidemiology
  • Zoonoses / microbiology*
  • Zoonoses / transmission