DNA extracellular traps are part of the immune repertoire of Periplaneta americana

M T C Nascimento; K P Silva; M C F Garcia; M N Medeiros; E A Machado; S B Nascimento; E M Saraiva

doi:10.1016/j.dci.2018.01.012

DNA extracellular traps are part of the immune repertoire of Periplaneta americana

Dev Comp Immunol. 2018 Jul:84:62-70. doi: 10.1016/j.dci.2018.01.012. Epub 2018 Jan 31.

Authors

M T C Nascimento¹, K P Silva², M C F Garcia², M N Medeiros³, E A Machado⁴, S B Nascimento³, E M Saraiva⁵

Affiliations

¹ Laboratório de Imunobiologia das Leishmanioses, Departamento de Imunologia, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, RJ Brazil; Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular (INCT-EM), Rio de Janeiro, Brazil.
² Laboratório de Imunobiologia das Leishmanioses, Departamento de Imunologia, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, RJ Brazil.
³ Laboratório de Bioquímica de insetos e Parasitos (Labip), Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
⁴ Laboratório de Bioquímica de insetos e Parasitos (Labip), Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil; Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular (INCT-EM), Rio de Janeiro, Brazil.
⁵ Laboratório de Imunobiologia das Leishmanioses, Departamento de Imunologia, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, RJ Brazil. Electronic address: [email protected].

PMID: 29391174
DOI: 10.1016/j.dci.2018.01.012

Abstract

Extracellular traps (ETs), web-like structures composed of DNA and histones, are released by innate immune cells in a wide range of organisms. ETs capture microorganisms, thereby avoiding their spread, and also concentrate antimicrobial molecules, which helps to kill microbes. Although vertebrate innate immune systems share homology with the insect immune system, ETosis have yet to be characterized in insects. Here, we report that the hemocytes of the hemimetabolous insect Periplaneta americana release ETs upon in vitro stimulation. We further discuss the relationship between ETs and nodulation and in controlling bacterial spread in vivo.

Keywords: Extracellular traps; Hemocyte; Innate immunity; Periplaneta amerciana.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cells, Cultured
DNA / genetics*
Escherichia coli / physiology*
Escherichia coli Infections / immunology*
Extracellular Traps / genetics*
Extracellular Traps / metabolism
Hemocytes / physiology*
Histones / metabolism
Immunity, Innate
Insect Proteins / metabolism
Microscopy, Electron
Periplaneta / immunology*

Substances

Histones
Insect Proteins
DNA