Infection-prevention on Ti implants by controlled drug release from folic acid/ZnO quantum dots sealed titania nanotubes

Mater Sci Eng C Mater Biol Appl. 2018 Apr 1:85:214-224. doi: 10.1016/j.msec.2017.12.034. Epub 2018 Jan 4.

Abstract

Bacterial infections and related complications are predominantly responsible for the failure of artificial biomaterials assisted tissue regeneration in clinic. In this work, a hybrid surface system is applied to prolong the drug release duration from dug-loaded titania nanotubes and thus to prevent Ti implants-associated bacterial infections. This feature is endowed by conjugating folic acid (FA) onto the surface of ZnO quantum dots (QDs)-NH2 via an amidation reaction. Titania nanotubes (TNTs) loaded with vancomycin (Van) are capped by these FA functionalized ZnO (ZnO-FA) QDs that keep stable in normal physiological environments but dissolves to Zn2+ in the mildly acidic environment after bacterial infections as validated by the drug release profile. The antibacterial ratio of TNTs-Van@ZnO-FA QDs against Staphylococcus aureus is enhanced from 60.8% to 98.8%while this value is only increased from 85.2% to 95.1% for TNTs-Van once the pH value of the environment is decreased from 7.4 to 5.5. This is due to the synergistic effects of Van and Zn2+ because the gradual dissolution of ZnO-FA caps on TNTs with the decrease of pH value can induce the acceleration of both Van and Zn2+ release. In addition, this TNTs-Van@ZnO-FA system also exhibits excellent biocompatibility because of the folic acid and sustained release of Zn ions. Hence, this surface system can be potentially used as a promising bioplatform on Ti-based metallic implants to prevent bacterial infection with a long-lasting effect.

Keywords: Antibacterial; Drug delivery; Infection prevention; Quantum dots; ZnO; pH sensitivity.

MeSH terms

  • Animals
  • Anti-Bacterial Agents / pharmacology
  • Cell Death / drug effects
  • Cell Line
  • Delayed-Action Preparations
  • Drug Liberation*
  • Folic Acid / chemistry*
  • Hydrogen-Ion Concentration
  • Mice
  • Microbial Sensitivity Tests
  • Microbial Viability / drug effects
  • Nanotubes / chemistry*
  • Nanotubes / ultrastructure
  • Prosthesis-Related Infections / microbiology
  • Prosthesis-Related Infections / pathology
  • Prosthesis-Related Infections / prevention & control*
  • Quantum Dots / chemistry*
  • Quantum Dots / ultrastructure
  • Spectrophotometry, Ultraviolet
  • Spectroscopy, Fourier Transform Infrared
  • Staphylococcus aureus / drug effects
  • Titanium / chemistry*
  • Vancomycin / pharmacology
  • Zinc Oxide / chemistry*

Substances

  • Anti-Bacterial Agents
  • Delayed-Action Preparations
  • titanium dioxide
  • Vancomycin
  • Folic Acid
  • Titanium
  • Zinc Oxide