Simultaneous demonstration of 3 synaptic markers: nerve endings, localization of AChE and AChR clusters at neuromuscular junctions of the rat in vitro

Biol Cell. 1986;56(2):181-3. doi: 10.1111/j.1768-322x.1986.tb00451.x.

Abstract

Our triple labelling method allows the AChE and AChR distributions and the nerve endings to be stained in the same preparation. This method which provides a detailed visualization of the synaptic contacts and is capable of revealing a weak AChE activity is particularly valuable in studies of synaptogenesis.

MeSH terms

  • Acetylcholinesterase / analysis*
  • Animals
  • Cells, Cultured
  • Fluorescent Antibody Technique
  • Histocytochemistry
  • Nerve Endings / enzymology
  • Nerve Endings / metabolism*
  • Neuromuscular Junction / embryology
  • Neuromuscular Junction / enzymology
  • Neuromuscular Junction / metabolism*
  • Rats
  • Receptors, Cholinergic / analysis*
  • Synapses / enzymology
  • Synapses / metabolism*

Substances

  • Receptors, Cholinergic
  • Acetylcholinesterase