Establishment and characterization of a human embryonic stem cell line, NERCe002-A-3, with inducible 14-3-3ζ overexpression

Jingjing He; Xingxiang Duan; Wen Li; Yingying Peng; Juan Yu; Liang Hu; Sicong Zeng; Yang Wang; Guangxiu Lu; Ge Lin; Yi Sun

doi:10.1016/j.scr.2018.01.017

Establishment and characterization of a human embryonic stem cell line, NERCe002-A-3, with inducible 14-3-3ζ overexpression

Stem Cell Res. 2018 Apr:28:11-15. doi: 10.1016/j.scr.2018.01.017. Epub 2018 Feb 28.

Authors

Jingjing He¹, Xingxiang Duan¹, Wen Li², Yingying Peng¹, Juan Yu³, Liang Hu⁴, Sicong Zeng⁵, Yang Wang¹, Guangxiu Lu⁶, Ge Lin⁷, Yi Sun⁸

Affiliations

¹ Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China.
² Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China; Key Laboratory of Stem Cells and Reproductive Engineering, Ministry of Health, Changsha, China; Reproductive & Genetic Hospital of CITIC-Xiangya, Changsha, China.
³ National Engineering and Research Center of Human Stem Cells, Changsha, China.
⁴ Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China; National Engineering and Research Center of Human Stem Cells, Changsha, China; Key Laboratory of Stem Cells and Reproductive Engineering, Ministry of Health, Changsha, China.
⁵ Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China; National Engineering and Research Center of Human Stem Cells, Changsha, China.
⁶ National Engineering and Research Center of Human Stem Cells, Changsha, China; Key Laboratory of Stem Cells and Reproductive Engineering, Ministry of Health, Changsha, China; Reproductive & Genetic Hospital of CITIC-Xiangya, Changsha, China.
⁷ Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China; National Engineering and Research Center of Human Stem Cells, Changsha, China; Key Laboratory of Stem Cells and Reproductive Engineering, Ministry of Health, Changsha, China; Reproductive & Genetic Hospital of CITIC-Xiangya, Changsha, China.
⁸ Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China; National Engineering and Research Center of Human Stem Cells, Changsha, China; Key Laboratory of Stem Cells and Reproductive Engineering, Ministry of Health, Changsha, China. Electronic address: [email protected].

PMID: 29414411
DOI: 10.1016/j.scr.2018.01.017

Abstract

NERCe002-A-3 cells were generated from the normal human embryonic stem cell line NERCe002-A. NERCe002-A-3 cells overexpressed 14-3-3ζ after exposure to doxycycline. 14-3-3ζ protein have the ability to bind a multitude of functionally diverse signalling proteins. The NERCe002-A-3 cell line is considered a model for functional studies of the 14-3-3ζ protein in hESC self-renewal and cell differentiation. Doxycycline-treated NERCe002-A-3 cells showed a>27-fold increase in relative expression of 14-3-3ζ as compared with un-induced cells. Characterization assays proved that NERCe002-A-3 cells express typical markers of pluripotency and have the ability to form the three germ layers in vivo.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

14-3-3 Proteins / metabolism*
Animals
Cell Culture Techniques / methods*
Cell Line
Human Embryonic Stem Cells / cytology*
Human Embryonic Stem Cells / metabolism*
Humans
Male
Mice
Reproducibility of Results

Substances

14-3-3 Proteins