Antiphotoaging Effect of Prunus yeonesis Blossom Extract via Inhibition of MAPK/AP-1 and Regulation of the TGF-βI/Smad and Nrf2/ARE Signaling Pathways

Photochem Photobiol. 2018 Jul;94(4):725-732. doi: 10.1111/php.12894. Epub 2018 Mar 25.

Abstract

Cherry blossoms have attracted attention as an ingredient with potential for use in skincare products. However, no skin photoaging-related research has been performed with this plant. In this study, cherry blossom extract (CBE) at 1, 10 and 100 μg mL-1 was investigated for its skin antiphotoaging effects in UVB-irradiated normal human dermal fibroblasts (NHDF) cells in vitro. Our results showed that CBE markedly increased type-I procollagen during UVB exposure via two pathways. Firstly, transcription activator protein-1 expression and MAP kinases were downregulated, consequently reducing the production of matrix metalloproteinase (MMP)-1 and MMP-3. Secondly, transforming growth factor TGF-βI secretion was upregulated by Smads. Application of CBE facilitated the nuclear translocation of Nrf2 against reactive oxygen species (ROS)-induced damage, which is essential for the coordinated induction of cytoprotective enzymes. Together, our findings suggest that CBE may be a promising ingredient for skin aging therapy and provide a novel approach for alleviating cutaneous aging.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Nucleus / metabolism
  • Cells, Cultured
  • Collagen Type I / metabolism
  • Flowers / chemistry*
  • Free Radical Scavengers / metabolism
  • Humans
  • Matrix Metalloproteinase 1 / genetics
  • Matrix Metalloproteinase 1 / metabolism
  • Matrix Metalloproteinase 3 / genetics
  • Matrix Metalloproteinase 3 / metabolism
  • Mitogen-Activated Protein Kinases / antagonists & inhibitors
  • NF-E2-Related Factor 2 / metabolism
  • Phosphorylation
  • Plant Extracts / pharmacology*
  • Procollagen / metabolism
  • Prunus / chemistry*
  • RNA, Messenger / metabolism
  • Reactive Oxygen Species / metabolism
  • Signal Transduction / drug effects*
  • Skin / cytology
  • Skin / drug effects*
  • Skin / enzymology
  • Skin / metabolism
  • Skin Aging / drug effects*
  • Smad Proteins / metabolism
  • Transcription Factor AP-1 / antagonists & inhibitors
  • Transforming Growth Factor beta1 / metabolism

Substances

  • Collagen Type I
  • Free Radical Scavengers
  • NF-E2-Related Factor 2
  • NFE2L2 protein, human
  • Plant Extracts
  • Procollagen
  • RNA, Messenger
  • Reactive Oxygen Species
  • Smad Proteins
  • Transcription Factor AP-1
  • Transforming Growth Factor beta1
  • Mitogen-Activated Protein Kinases
  • MMP3 protein, human
  • Matrix Metalloproteinase 3
  • MMP1 protein, human
  • Matrix Metalloproteinase 1