Loss of function of a rice TPR-domain RNA-binding protein confers broad-spectrum disease resistance

Xiaogang Zhou; Haicheng Liao; Mawsheng Chern; Junjie Yin; Yufei Chen; Jianping Wang; Xiaobo Zhu; Zhixiong Chen; Can Yuan; Wen Zhao; Jing Wang; Weitao Li; Min He; Bingtian Ma; Jichun Wang; Peng Qin; Weilan Chen; Yuping Wang; Jiali Liu; Yangwen Qian; Wenming Wang; Xianjun Wu; Ping Li; Lihuang Zhu; Shigui Li; Pamela C Ronald; Xuewei Chen

doi:10.1073/pnas.1705927115

Loss of function of a rice TPR-domain RNA-binding protein confers broad-spectrum disease resistance

Proc Natl Acad Sci U S A. 2018 Mar 20;115(12):3174-3179. doi: 10.1073/pnas.1705927115. Epub 2018 Feb 5.

Authors

Xiaogang Zhou^{1

2

3

4}, Haicheng Liao^{1

2

3}, Mawsheng Chern^{5

6}, Junjie Yin^{1

2

3}, Yufei Chen^{1

2

3}, Jianping Wang^{1

2

3}, Xiaobo Zhu^{1

2

3}, Zhixiong Chen^{1

2

3}, Can Yuan^{1

2

3}, Wen Zhao^{1

2

3}, Jing Wang^{1

2

3}, Weitao Li^{1

2

3}, Min He^{1

2

3}, Bingtian Ma^{1

2

3}, Jichun Wang^{1

2

3}, Peng Qin^{1

2

3}, Weilan Chen^{1

2

3}, Yuping Wang^{1

2

3}, Jiali Liu^{1

2

3}, Yangwen Qian⁷, Wenming Wang^{1

2

3}, Xianjun Wu^{1

2

3}, Ping Li^{1

2

3}, Lihuang Zhu^{8

9}, Shigui Li^{1

2

3}, Pamela C Ronald^{5

6}, Xuewei Chen^{10

2

3}

Affiliations

¹ Key Laboratory of Major Crop Diseases, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan 611130, China.
² State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan 611130, China.
³ Collaborative Innovation Center for Hybrid Rice in Yangtze River Basin, Rice Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan 611130, China.
⁴ Key Laboratory for Aging and Regenerative Medicine, Department of Pharmacology, School of Pharmacy, Southwest Medical University, Luzhou, Sichuan 646000, China.
⁵ Department of Plant Pathology, University of California, Davis, CA 95616.
⁶ The Genome Center, University of California, Davis, CA 95616.
⁷ Biogle Genome Editing Center, Changzhou, Jiangsu 213125, China.
⁸ State Key Laboratory of Plant Genomics, Chinese Academy of Sciences, Beijing 100101, China.
⁹ National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
¹⁰ Key Laboratory of Major Crop Diseases, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan 611130, China; [email protected].

Abstract

Crops carrying broad-spectrum resistance loci provide an effective strategy for controlling infectious disease because these loci typically confer resistance to diverse races of a pathogen or even multiple species of pathogens. Despite their importance, only a few crop broad-spectrum resistance loci have been reported. Here, we report the identification and characterization of the rice bsr-k1 (broad-spectrum resistance Kitaake-1) mutant, which confers broad-spectrum resistance against Magnaporthe oryzae and Xanthomonas oryzae pv oryzae with no major penalty on key agronomic traits. Map-based cloning reveals that Bsr-k1 encodes a tetratricopeptide repeats (TPRs)-containing protein, which binds to mRNAs of multiple OsPAL (OsPAL1-7) genes and promotes their turnover. Loss of function of the Bsr-k1 gene leads to accumulation of OsPAL1-7 mRNAs in the bsr-k1 mutant. Furthermore, overexpression of OsPAL1 in wild-type rice TP309 confers resistance to M. oryzae, supporting the role of OsPAL1 Our discovery of the bsr-k1 allele constitutes a significant conceptual advancement and provides a valuable tool for breeding broad-spectrum resistant rice.

Keywords: TPR protein; blast disease; broad-spectrum resistance; innate immunity; rice.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Cytoplasm / metabolism
Disease Resistance / genetics
Gene Expression Regulation, Plant
Magnaporthe / pathogenicity
Mutation
Oryza / genetics
Oryza / microbiology
Oryza / physiology*
Plant Diseases / genetics*
Plant Diseases / microbiology
Plant Proteins / genetics*
Plant Proteins / metabolism
Plants, Genetically Modified / genetics
Protein Domains
RNA, Messenger / metabolism
RNA-Binding Proteins / genetics*
RNA-Binding Proteins / metabolism
Repetitive Sequences, Amino Acid
Xanthomonas / pathogenicity

Substances

Plant Proteins
RNA, Messenger
RNA-Binding Proteins

Grants and funding

R01 GM122968/GM/NIGMS NIH HHS/United States