Quantitative Analysis of Precursors MicroRNAs and Their Respective Mature MicroRNAs in Cancer Exosomes Overtime

Nuno Bastos; Sónia A Melo

doi:10.1007/978-1-4939-7601-0_11

Quantitative Analysis of Precursors MicroRNAs and Their Respective Mature MicroRNAs in Cancer Exosomes Overtime

Methods Mol Biol. 2018:1733:137-143. doi: 10.1007/978-1-4939-7601-0_11.

Authors

Nuno Bastos^{1

2}, Sónia A Melo^{3

4

5}

Affiliations

¹ Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal.
² Institute of Molecular Pathology and Immunology, University of Porto (IPATIMUP), Porto, Portugal.
³ Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal. [email protected].
⁴ Institute of Molecular Pathology and Immunology, University of Porto (IPATIMUP), Porto, Portugal. [email protected].
⁵ Medical Faculty, University of Porto, Porto, Portugal. [email protected].

PMID: 29435929
DOI: 10.1007/978-1-4939-7601-0_11

Abstract

Exosomes are a cell-to-cell communication system that transports information to neighbor and/or distant cells. Their content is composed of lipids, proteins, RNAs, and DNA. The role of exosomes released by cancer cells in disease progression has been widely studied. Here, we report a detailed protocol to quantify precursor microRNAs and mature microRNAs overtime in exosomes of cancer cells. Those precursor microRNAs that are packaged together with RISC proteins will get further processed in the exosomes overtime and an accumulation of the mature form of the microRNAs will occur.

Keywords: Exosomes; Exosomes isolation; MicroRNAs; Pre-microRNAs; qPCR.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Line
Humans
MicroRNAs / genetics*
Neoplasms / genetics*
Neoplasms / metabolism*
RNA Precursors / genetics*
Real-Time Polymerase Chain Reaction
Subcellular Fractions

Substances

MicroRNAs
RNA Precursors