Fast and efficient genetic engineering of hematopoietic precursor cells for the study of dendritic cell migration

Eur J Immunol. 2018 Jun;48(6):1074-1077. doi: 10.1002/eji.201747358. Epub 2018 Mar 1.

Abstract

Estrogen inducible Hoxb8 leads to conditional immortalization of hematopoietic precursors. These cells can be cultured and infected with the CRISPR/Cas9 system for genome editing, circumventing resource consuming generation of mouse models. The resultant cells retain their ability to differentiate into migratory dendritic cells.

Keywords: CCL19; CCR7; CRISPR/Cas9; Cell migration; Genome editing.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CRISPR-Cas Systems
  • Cell Differentiation
  • Cell Line, Transformed
  • Cell Movement / genetics*
  • Cell Self Renewal / genetics
  • Dendritic Cells / physiology*
  • Estrogens / metabolism
  • Gene Editing
  • Genetic Engineering / methods*
  • Hematopoietic Stem Cells / physiology*
  • Homeodomain Proteins / genetics*
  • Humans
  • Mice
  • Models, Animal

Substances

  • Estrogens
  • Homeodomain Proteins
  • Hoxb8 protein, mouse