Overexpression of PtABCC1 contributes to mercury tolerance and accumulation in Arabidopsis and poplar

Biochem Biophys Res Commun. 2018 Mar 18;497(4):997-1002. doi: 10.1016/j.bbrc.2018.02.133. Epub 2018 Feb 16.

Abstract

Mercury (Hg) is a highly biotoxic heavy metal that contaminates the environment. Phytoremediation is a green technology for environmental remediation and is used to clean up Hg contaminated soil in recent years. In this study, we isolated an ATP-binding cassette (ABC) transporter gene PtABCC1 from Populus trichocarpa and overexpressed it in Arabidopsis and poplar. The transgenic plants conferred higher Hg tolerance than wild type (WT) plants, and overexpression of PtABCC1 could lead to 26-72% or 7-160% increase of Hg accumulation in Arabidopsis or poplar plants, respectively. These results demonstrated that PtABCC1 plays a crucial role in enhancing tolerance and accumulation to Hg in plants, which provides a promising way for phytoremediation of Hg contamination.

Keywords: Accumulation; Hg; Poplar; PtABCC1; Tolerance.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptation, Biological / genetics*
  • Arabidopsis / genetics
  • Biodegradation, Environmental / drug effects*
  • Mercury / pharmacology*
  • Multidrug Resistance-Associated Proteins / genetics
  • Multidrug Resistance-Associated Proteins / physiology*
  • Plant Proteins / genetics
  • Plant Proteins / metabolism
  • Plants, Genetically Modified / metabolism
  • Plants, Genetically Modified / physiology*
  • Populus / genetics

Substances

  • Multidrug Resistance-Associated Proteins
  • Plant Proteins
  • Mercury
  • multidrug resistance-associated protein 1