Evaluation of mosquito larvicidal activity of fruit extracts of Acacia auriculiformis against the Japanese encephalitis vector Culex vishnui

Nat Prod Res. 2019 Jun;33(11):1682-1686. doi: 10.1080/14786419.2018.1428585. Epub 2018 Feb 19.

Abstract

The larvicidal potentiality of crude and ethyl acetate extracts of fruits of Acacia auriculiformis was investigated against all the larval instars of JE vector Culex vishnui. The crude extracts showed good results against all the larval instars with highest mortality at 0.09%. Highest mortality was found at 300 ppm of ethyl acetate extract. Lowest LC50 value was obtained at 72 h for third instar larvae. Non target organisms tested, showed no to very less mortality to ethyl acetate solvent extract. Presence of N-H stretching, a C=O stretching, C=C and C-N stretching vibrations of secondary amide or amine group were confirmed from IR analysis. GC-MS analysis revealed the presence of three compounds namely Ethane 2-chloro-1,1-dimethoxy, Acetic acid, 1-methyl ether ester and [4-[1-[3,5-Dimethyl-4[(trimethylsilyl)oxy)phenyl]-1,3-dimethylbutyl)-2,6dimethylphenoxy)(trimethyl) silane, responsible for mosquito larval death.

Keywords: active ingredient; larvicidal activity; non-target organisms.

MeSH terms

  • Acacia / chemistry*
  • Acetates / chemistry
  • Animals
  • Culex / drug effects*
  • Encephalitis, Japanese / transmission
  • Fruit / chemistry
  • Gas Chromatography-Mass Spectrometry
  • Insecticides / chemistry
  • Insecticides / pharmacology*
  • Larva / drug effects
  • Lethal Dose 50
  • Mosquito Vectors / drug effects
  • Plant Extracts / chemistry
  • Plant Extracts / pharmacology*
  • Plant Leaves / chemistry

Substances

  • Acetates
  • Insecticides
  • Plant Extracts
  • ethyl acetate