FACS single cell index sorting is highly reliable and determines immune phenotypes of clonally expanded T cells

Eur J Immunol. 2018 Jul;48(7):1248-1250. doi: 10.1002/eji.201847507. Epub 2018 Apr 3.

Abstract

FACS index sorting allows the isolation of single cells with retrospective identification of each single cell's high-dimensional immune phenotype. We experimentally determine the error rate of index sorting and combine the technology with T cell receptor sequencing to identify clonal T cell expansion in aplastic anemia bone marrow as an example.

Keywords: Aplastic anemia; Index sorting; Multicolor flow cytometry; Single cell technologies; T cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anemia, Aplastic / diagnosis*
  • Bone Marrow / pathology*
  • Cell Proliferation
  • Cell Separation
  • Clone Cells
  • Diagnostic Errors / prevention & control*
  • Flow Cytometry / methods*
  • Humans
  • Immunity, Cellular
  • Phenotype
  • Single-Cell Analysis
  • T-Lymphocytes / pathology*