Three new sesquiterpenes from Ainsliaea glabra

Nat Prod Res. 2019 Jan;33(2):274-279. doi: 10.1080/14786419.2018.1446134. Epub 2018 Mar 14.

Abstract

Three new sesquiterpenes: 4-acrylic-6-methyl-α-tetralone (1), ainsliaea acid A (2) and ainsliaea acid B (3), together with 8 known compounds (4-11) were isolated from the whole herb of Ainsliaea glabra and their structures were established by means of 1D and 2D NMR spectroscopy and HR-ESIMS. Compounds 1-6 were tested for the inhibition of nuclear factor kappa B (NF-κB) in the 293-NF-κB-luciferase reporter cell line induced by lipopolysaccharide (LPS), and compound 2 was further tested for the production of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), IL-6 and IL-10 in RAW264.7 macrophages induced by LPS. The isolated compound 2 exhibited significant anti-inflammatory activity.

Keywords: anti-inflammatory activity; sesquiterpene.

MeSH terms

  • Animals
  • Anti-Inflammatory Agents / chemistry
  • Anti-Inflammatory Agents / isolation & purification*
  • Anti-Inflammatory Agents / pharmacology
  • Asteraceae / chemistry*
  • Cytokines / drug effects
  • Lipopolysaccharides / pharmacology
  • Macrophages / metabolism
  • Mice
  • Molecular Structure
  • NF-kappa B / antagonists & inhibitors
  • Plant Extracts / chemistry
  • RAW 264.7 Cells
  • Sesquiterpenes / chemistry
  • Sesquiterpenes / isolation & purification*
  • Sesquiterpenes / pharmacology

Substances

  • Anti-Inflammatory Agents
  • Cytokines
  • Lipopolysaccharides
  • NF-kappa B
  • Plant Extracts
  • Sesquiterpenes