To investigate the expression of H19 long non-coding RNA (lncRNA) and zinc finger E-box-binding protein 1 (ZEB1) in the trophoblast of women with spontaneous abortion. Methods: A total of 20 women underwent miscarriage were enrolled as a miscarriage group, while 20 women underwent artificial abortion were enrolled as a control group. Reverse transcriptional fluorescence quantitative polymerase chain reaction (RT-qPCR) was used to detect the expression of H19 lncRNA and miR-200, and Western blot was used to detect the expression of ZEB1. Results: Compared to the control group, a tendency of decreased H19 was detected in the miscarriage group, with no significant difference (1.28±0.08 vs 1.66±0.21, P=0.091). There was no significant difference in miR-200 between the 2 groups (0.75±0.35 vs 0.58±0.33, P=0.0.533). The expression of ZEB1 was significantly decreased in the miscarriage group compared to that in the control group (0.22±0.03 vs 0.41±0.04, P=0.0003). The expression of H19 lncRNA and ZEB1 was positively correlated (r=0.529, P=0.0005), and the correlation between H19 lncRNA and miR-200 had no statistical significance (r=0.0293, P=0.858). The correlation between miR-200 and ZEB1 also had no statistical significance (r=-0.132, P=0.416). Conclusion: Down-regulation of ZEB1 in the trophoblast might be related to miscarriage. H19 lncRNA may regulate the expression of ZEB1.
目的：探讨长链非编码RNA H19(long non-coding RNA H19，H19 lncRNA)及E盒锌指结合蛋白1(zinc finger E-box-binding protein 1，ZEB1)在自然流产患者绒毛组织中的表达及意义。方法：采用反转录荧光定量PCR(reverse transcriptional quantitative polymerase chain reaction，RT-qPCR)技术及Western印迹分别检测20例自然流产患者(自然流产组)和20例正常早孕行人工流产妇女(对照组)绒毛组织中H19 lncRNA，miR-200及ZEB1的表达。结果：与对照组相比，自然流产组绒毛组织中H19 lncRNA的表达具有下降趋势，但差异无统计学意义(1.28±0.08 vs 1.66±0.21，P=0.091)；miR-200在两组中表达差异无统计学意义(0.75±0.35 vs 0.58±0.33，P=0.129)；自然流产组绒毛组织中ZEB1的表达量明显低于对照组，差异具有统计学意义(0.22±0.03 vs 0.41±0.04，P=0.0003)。H19 lncRNA与ZEB1表达水平呈中度正相关(r=0.529，P=0.0005)，H19 lncRNA与miR-200(r=0.0293，P=0.858)及miR-200与ZEB1(r=–0.132，P=0.416)无明显相关性。结论：自然流产胚胎绒毛组织中ZEB1的表达下调可能与自然流产相关，H19 lncRNA可能参与调控ZEB1的表达。.