Abstract
Mitochondrial dysfunction, and consequently altered aerobic energy metabolism, is associated with numerous retinal diseases, including photoreceptor degeneration and diabetic retinopathy. Here, we describe a detailed protocol to directly measure oxygen consumption in the intact retina ex vivo using microplate-based fluorescence technology. We have used this method to assess preferred energy substrate for retinal tissue and suggested its application for investigating mechanisms of retinal disease.
Keywords:
Aerobic respiration; Energy metabolism; Mouse models; Photoreceptor; Retinal degeneration.
Publication types
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Research Support, N.I.H., Intramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Basic-Leucine Zipper Transcription Factors / genetics
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Biological Assay / instrumentation
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Biological Assay / methods*
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Cell Respiration
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Disease Models, Animal
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Eye Proteins / genetics
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Humans
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Mice
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Mice, Inbred C57BL
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Mice, Transgenic
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Mitochondria / metabolism*
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Oxygen / analysis*
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Oxygen / metabolism
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Oxygen Consumption
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Retina / cytology
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Retina / metabolism*
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Retinal Diseases / genetics
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Retinal Diseases / pathology*
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Spectrometry, Fluorescence / instrumentation
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Spectrometry, Fluorescence / methods
Substances
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Basic-Leucine Zipper Transcription Factors
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Eye Proteins
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Nrl protein, mouse
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Oxygen