Expression of Genes Associated with Telomere Homeostasis in TP53 Mutant LoVo Cell Lines as a Model for Genomic Instability

Oumar Samassekou; Nathalie Bastien; Ju Yan; Sabine Mai; Régen Drouin

doi:10.1007/978-1-4939-7780-2_16

Expression of Genes Associated with Telomere Homeostasis in TP53 Mutant LoVo Cell Lines as a Model for Genomic Instability

Methods Mol Biol. 2018:1769:253-262. doi: 10.1007/978-1-4939-7780-2_16.

Authors

Oumar Samassekou¹, Nathalie Bastien², Ju Yan³, Sabine Mai⁴, Régen Drouin⁵

Affiliations

¹ 3D Signatures Holdings Inc. MaRS Centre, Toronto, ON, Canada.
² Laboratoire D'anatomopathologie et de Cytologie, Laboratoires médicaux de la Capitale Nationale et des Îles, Québec, QC, Canada.
³ Cytogenetics and Molecular Cytogenetics Laboratory, Beijing Boren Hospital, Beijing, China.
⁴ Department of Physiology and Pathophysiology, Cell Biology, Research Institute of Oncology and Hematology, CancerCare Manitoba, University of Manitoba, Winnipeg, MB, Canada.
⁵ Division of Medical Genetics, Department of Pediatrics, Laval University and Centre hospitalier universitaire de Québec, Quebec City, QC, Canada. [email protected].

PMID: 29564829
DOI: 10.1007/978-1-4939-7780-2_16

Abstract

We describe a method that assesses the impact of specific mutations of TP53 and genomic instability on gene expression of the most important genes involved in telomere length and structure homeostasis. The approaches consist of using a reverse transcriptase method and a quantitative PCR that were applied to isogenic cell lines from a colon cancer.

Keywords: Genomic instability; Quantitative PCR; Reverse transcriptase; Shelterin; Telomerase; Telomere; Telomere-associated gene.

MeSH terms

Cell Line
Gene Expression Regulation*
Genomic Instability*
Humans
Mutation*
Telomere / genetics*
Telomere / metabolism
Telomere Homeostasis / genetics*
Tumor Suppressor Protein p53 / genetics*
Tumor Suppressor Protein p53 / metabolism

Substances

Tumor Suppressor Protein p53