Whole-genome sequencing in the investigation of recurrent invasive group A streptococcus outbreaks in a maternity unit

J Hosp Infect. 2019 Mar;101(3):320-326. doi: 10.1016/j.jhin.2018.03.018. Epub 2018 Mar 22.

Abstract

Background: The clinical manifestations of group A streptococcus (GAS) (Streptococcus pyogenes) are diverse, ranging from asymptomatic colonization to devastating invasive disease. Maternity-related clusters of invasive GAS (iGAS) infection are complex to investigate and control, especially if recurrent.

Aim: To investigate three episodes of emm 75 GAS/iGAS infection in maternity patients at one hospital site over a four-year period (two with monophyletic ancestry).

Methods: The episodes are described, together with whole-genome sequence (WGS) isolate analyses. Single nucleotide polymorphism differences were compared with contemporaneous emm 75 genomes.

Findings: Over the four-year study period, seven mothers had emm 75 GAS/iGAS and one mother had emm 3 iGAS (in year 4) (subsequently discounted as linked). Three (clinical/screening samples) of the seven babies of emm-75-positive mothers and three screened healthcare workers were positive for emm 75 GAS. WGS similarity suggested a shared ancestral lineage and a common source transmission, but directionality of transmission cannot be inferred. However, the findings indicate that persistence of a particular clone in a given setting may be long term.

Conclusions: Occupational health procedures were enhanced, staff were screened, and antibiotic therapy was provided to GAS-positive staff and patients. The definitive source of infection could not be identified, although staff-patient transmission was the most likely route. The pattern of clonal GAS transmission over the four-year study period suggests that long-term persistence of GAS may have occurred.

Keywords: Maternity; Streptococcus spp.; Whole-genome sequencing; iGAS.

MeSH terms

  • Adult
  • Cluster Analysis
  • Disease Outbreaks*
  • Disease Transmission, Infectious*
  • Female
  • Genotype
  • Health Personnel
  • Hospitals, Maternity
  • Humans
  • Infant
  • Infant, Newborn
  • Molecular Epidemiology
  • Molecular Typing
  • Mothers
  • Polymorphism, Single Nucleotide
  • Streptococcal Infections / epidemiology*
  • Streptococcal Infections / microbiology
  • Streptococcal Infections / transmission
  • Streptococcus pyogenes / classification*
  • Streptococcus pyogenes / genetics
  • Streptococcus pyogenes / isolation & purification*
  • Whole Genome Sequencing*