Abstract
Noninvasive screening methods for the detection of colorectal cancers (CRC) at curable stages rely on the identification of specific biomarkers. Our group has shown that mRNA stool assays represent a powerful and robust approach for the prediction of colorectal neoplasms. In this methodological chapter, we describe the procedures to isolate good quality stool RNA and the steps to evaluate the levels of specific host mRNA markers such as ITGA6, MYC, and GADD45B using TaqMan-based quantitative and droplet digital PCR approaches.
Keywords:
Colorectal cancer; Diagnosis; Stool; ddPCR; mRNA; qPCR.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antigens, Differentiation / genetics
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Biomarkers, Tumor / analysis*
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Colorectal Neoplasms / diagnosis*
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Early Detection of Cancer / instrumentation
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Early Detection of Cancer / methods*
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Feces / chemistry*
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Humans
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Integrin alpha6 / genetics
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Proto-Oncogene Proteins c-myc / genetics
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RNA, Messenger / analysis*
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Real-Time Polymerase Chain Reaction / instrumentation
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Real-Time Polymerase Chain Reaction / methods
Substances
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Antigens, Differentiation
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Biomarkers, Tumor
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GADD45B protein, human
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ITGA6 protein, human
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Integrin alpha6
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MYC protein, human
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Proto-Oncogene Proteins c-myc
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RNA, Messenger