Discovery, gene modification, and optimization of fermentation of an enduracidin-producing strain

J Asian Nat Prod Res. 2018 Jul;20(7):633-648. doi: 10.1080/10286020.2018.1451517. Epub 2018 Mar 28.

Abstract

Enduracidin significantly inhibits Gram-positive bacteria and had been widely used in many fields. However, as the poor technology for production of enduracidin and its scarcity, identification of novel strategies for production of enduracidin is important. Our group developed two methods to improve the yield of the production of enduracidin. The yield of enduracidin was increased by three- to fivefold. The highest yields of enduracidin A and enduracidin B achieved were 63.7 and 82.13 mg/ml. Thus, our results might provide a new reference method for the industrial production of enduracidin.

Keywords: Enduracidin; fermentation optimization; gene mutation; marine microorganisms.

MeSH terms

  • Amino Acids / pharmacology
  • Anti-Bacterial Agents / biosynthesis*
  • Anti-Bacterial Agents / chemistry
  • Anti-Bacterial Agents / pharmacology
  • Fermentation
  • Molecular Structure
  • Mutation
  • Nitrogen / metabolism
  • Peptides, Cyclic / biosynthesis*
  • Peptides, Cyclic / chemistry
  • Peptides, Cyclic / pharmacology
  • Streptomyces / drug effects
  • Streptomyces / genetics
  • Streptomyces / metabolism
  • Temperature

Substances

  • Amino Acids
  • Anti-Bacterial Agents
  • Peptides, Cyclic
  • enduracidin A
  • enduracidin B
  • Nitrogen