Proteobacteria and Bacteroidetes are major phyla of filterable bacteria passing through 0.22 μm pore size membrane filter, in Lake Sanaru, Hamamatsu, Japan

Biosci Biotechnol Biochem. 2018 Jul;82(7):1260-1263. doi: 10.1080/09168451.2018.1456317. Epub 2018 Mar 29.

Abstract

141 filterable bacteria that passed through a 0.22 μm pore size filter were isolated from Lake Sanaru in Hamamatsu, Japan. These belonged to Proteobacteria, Bacteroidetes, Firmicutes, or Actinobacteria among which the first two phyla comprised the majority of the isolates. 48 isolates (12 taxa) are candidates assignable to new bacterial species or genera of Proteobacteria or Bacteroidetes.

Keywords: COD: chemical oxygen demand; Filterable bacteria; LB: lysogeny broth; MB: marine broth; brackish lake; isolation; membrane filter.

MeSH terms

  • Actinobacteria / genetics
  • Actinobacteria / isolation & purification*
  • Bacteroidetes / classification
  • Bacteroidetes / genetics
  • Bacteroidetes / isolation & purification*
  • Filtration / methods*
  • Firmicutes / genetics
  • Firmicutes / isolation & purification*
  • Japan
  • Lakes / microbiology*
  • Membranes, Artificial*
  • Oxygen / metabolism
  • Phylogeny
  • Proteobacteria / classification
  • Proteobacteria / genetics
  • Proteobacteria / isolation & purification*
  • RNA, Ribosomal, 16S / genetics
  • Saline Waters
  • Water Microbiology

Substances

  • Membranes, Artificial
  • RNA, Ribosomal, 16S
  • Oxygen