Isolation of a cDNA probe for a human jejunal brush-border hydrolase, sucrase-isomaltase, and assignment of the gene locus to chromosome 3

Gene. 1987;57(1):101-10. doi: 10.1016/0378-1119(87)90181-8.

Abstract

We report the nucleotide sequence and derived amino acid sequence of a cDNA clone encoding most of the N-terminal, isomaltase region of human sucrase-isomaltase (SI). A plasmid containing this cDNA, pS12, identifies a 6-kb mRNA found in human jejunum and the human colon carcinoma cell line Caco-2. This human SI cDNA shows extensive overall homology with recently published rabbit SI cDNA. Using pS12 to probe DNA from a panel of somatic cell hybrids, we have assigned the gene encoding human SI to chromosome 3.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Bacteriophage lambda / genetics
  • Cell Line
  • Chromosomes, Human, Pair 3*
  • Cloning, Molecular
  • DNA / isolation & purification*
  • Escherichia coli / genetics
  • Genes*
  • Humans
  • Jejunum / enzymology*
  • Microvilli / enzymology*
  • Molecular Sequence Data
  • Multienzyme Complexes / genetics*
  • RNA, Messenger / genetics
  • Sequence Homology, Nucleic Acid
  • Species Specificity
  • Sucrase-Isomaltase Complex / genetics*
  • Transcription, Genetic

Substances

  • Multienzyme Complexes
  • RNA, Messenger
  • DNA
  • Sucrase-Isomaltase Complex

Associated data

  • GENBANK/M22616